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Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.

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Sutcliffe, J G·1978·Proceedings of the National Academy of Sciences

Abstract

I have determined the nucleotide sequence of the ampicillin resistance gene of pBR322, an Escherichia coli plasmid that encodes a penicillin beta-lactamase. This gene codes for a protein of 286 amino acid residues. The first 23 amino acids presumably form a signal for secretion, because they do not appear in the mature enzyme, whose partial amino acid sequence has been determined independently.

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Metadata

Authors
Sutcliffe, J. G.
Venue
Proceedings of the National Academy of Sciences
Published
1978-08
Volume
75
Issue
8
Pages
3737-3741
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2109068357
Cited by
782
References
0
FWCI
33.15
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. https://app.cochat.ai/papers/10.1073/pnas.75.8.3737
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Sutcliffe, J. G. (1978). Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.75.8.3737

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.