Novel Carbapenem-Hydrolyzing β-Lactamase, KPC-1, from a Carbapenem-Resistant Strain of Klebsiella pneumoniae
Open accessAbstract
A Klebsiella pneumoniae isolate showing moderate to high-level imipenem and meropenem resistance was investigated. The MICs of both drugs were 16 μg/ml. The β-lactamase activity against imipenem and meropenem was inhibited in the presence of clavulanic acid. The strain was also resistant to extended-spectrum cephalosporins and aztreonam. Isoelectric focusing studies demonstrated three β-lactamases, with pIs of 7.2 (SHV-29), 6.7 (KPC-1), and 5.4 (TEM-1). The presence of bla SHV and bla TEM genes was confirmed by specific PCRs and DNA sequence analysis. Transformation and conjugation studies with Escherichia coli showed that the β-lactamase with a pI of 6.7, KPC-1 ( K. pneumoniae carbapenemase-1), was encoded on an approximately 50-kb nonconjugative plasmid. The gene, bla KPC-1 , was cloned in E. coli and shown to confer resistance to imipenem, meropenem, extended-spectrum cephalosporins, and aztreonam. The amino acid sequence of the novel carbapenem-hydrolyzing β-lactamase, KPC-1, showed 45% identity to the pI 9.7 carbapenem-hydrolyzing β-lactamase, Sme-1, from Serratia marcescens S6. Hydrolysis studies showed that purified KPC-1 hydrolyzed not only carbapenems but also penicillins, cephalosporins, and monobactams. KPC-1 had the highest affinity for meropenem. The kinetic studies also revealed that clavulanic acid and tazobactam inhibited KPC-1. An examination of the outer membrane proteins of the parent K. pneumoniae strain demonstrated that the strain does not express detectable levels of OmpK35 and OmpK37, although OmpK36 is present. We concluded that carbapenem resistance in K. pneumoniae strain 1534 is mainly due to production of a novel Bush group 2f, class A, carbapenem-hydrolyzing β-lactamase, KPC-1, although alterations in porin expression may also play a role.
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KPC was first reported in 1996 from a K. pneumoniae recovered from a patient in North Carolina, USA.
“KPC was first reported in 1996 from a K. pneumoniae recovered from a patient in North Carolina, USA (25).”
From Mechanisms of Antibiotic Resistance (2016) · cites this paper as [25]
Cites
Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589 · see citing works
Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically
Ferraro, M. J. (2000) · Cited by 16,875 · see citing works
National Committee for Clinical Laboratory Standards
Bruck, E. (1980) · PEDIATRICS · doi:10.1542/peds.65.1.187 · Cited by 14,736 · see citing works
REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTION
Rosenberg, M. J., & Court, D. (1979) · Annual Review of Genetics · doi:10.1146/annurev.ge.13.120179.001535 · Cited by 2,726 · see citing works
A functional classification scheme for beta-lactamases and its correlation with molecular structure
Bush, K., Jacoby, G. A., & Medeiros, A. A. (1995) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.39.6.1211 · Cited by 2,485 · see citing works
Self-citationDeterminant of cistron specificity in bacterial ribosomes
Shine, J., & Dalgarno, L. (1975) · Nature · doi:10.1038/254034a0 · Cited by 1,365 · see citing works
A standard numbering scheme for the class A β -lactamases
Ambler, R. P., Coulson, A. F. W., Frère, J., Ghuysen, J., Joris, B., Forsman, M., Lévesque, R. C., Tiraby, G., & Waley, S. G. (1991) · Biochemical Journal · doi:10.1042/bj2760269 · Cited by 1,076 · see citing works
Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.
Sutcliffe, J. G. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.8.3737 · Cited by 784 · see citing works
Fridkin, S. K., Steward, C. D., Edwards, J. R., Pryor, E. R., McGowan, J. E., Archibald, L. K., Gaynes, R. P., & Tenover, F. C. (1999) · Clinical Infectious Diseases · doi:10.1086/520193 · Cited by 486 · see citing works
Self-citationEnterobacteriaceae : introduction and identification
Farmer, J. J. (1995) · Medical Entomology and Zoology · Cited by 474 · see citing works
Bradford, P. A., Urban, C. M., Mariano, N., Projan, S. J., Rahal, J. J., & Bush, K. (1997) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.41.3.563 · Cited by 450 · see citing works
Self-citationOSANO, E., Arakawa, Y., Wacharotayankun, R., et al. (1994) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.38.1.71 · Cited by 440 · see citing works
Archibald, L. K., Phillips, L., Monnet, D. L., McGowan, J. E., Tenover, F. C., & Gaynes, R. P. (1997) · Clinical Infectious Diseases · doi:10.1093/clinids/24.2.211 · Cited by 436 · see citing works
Self-citationKinship and Diversification of Bacterial Penicillin-Binding Proteins and β-Lactamases
Massova, I., & Mobashery, S. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.1.1 · Cited by 422 · see citing works
Carbapenem-hydrolyzing beta-lactamases
Rasmussen, B. A., & Bush, K. (1997) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.41.2.223 · Cited by 362 · see citing works
Self-citationJoris, B., Ghuysen, J., Dive, G., Renard, A., Dideberg, O., Charlier, P., Frère, J., Kelly, J. A., Boyington, J. C., & Moews, P. C. (1988) · Biochemical Journal · doi:10.1042/bj2500313 · Cited by 333 · see citing works
SYKES, R. B., BONNER, D. P., Bush, K., & Georgopapadakou, N. H. (1982) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.21.1.85 · Cited by 314 · see citing works
Self-citationA novel integron-like element carrying the metallo-beta-lactamase gene blaIMP
Arakawa, Y., Murakami, M. T., Suzuki, K. Z., et al. (1995) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.39.7.1612 · Cited by 311 · see citing works
Portnoy, D. A., Wolf‐Watz, H., Bölin, I., et al. (1984) · Infection and Immunity · doi:10.1128/iai.43.1.108-114.1984 · Cited by 284 · see citing works
Rasheed, J. K., Jay, C., Metchock, B. G., Berkowitz, F. E., Weigel, L. M., Crellin, J., Steward, C. D., Hill, B. C., Medeiros, A. A., & Tenover, F. C. (1997) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.41.3.647 · Cited by 274 · see citing works
Self-citationMartı́nez-Martı́nez, L., Pascual, Á., Hernández-Allés, S., Alvarez-Díaz, D., Suárez, A. I., Tran, J. H., Benedi, V. J., & Jacoby, G. A. (1999) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.43.7.1669 · Cited by 249 · see citing works
Ouellette, M., Bissonnette, L., & Roy, P. H. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.21.7378 · Cited by 239 · see citing works
Bauernfeind, A., Stemplinger, I., Jungwirth, R., Ernst, S., & Casellas, J. M. (1996) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.40.2.509 · Cited by 220 · see citing works
Rasmussen, B. A., Bush, K., Keeney, D. R., Yang, Y., Hare, R. S., O'Gara, C. J., & Medeiros, A. A. (1996) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.40.9.2080 · Cited by 217 · see citing works
Self-citationPorin expression in clinical isolates of Klebsiella pneumoniae
Hernández-Allés, S., Albertí, S., Álvarez, D., Doménech‐Sánchez, A., Martı́nez-Martı́nez, L., Gil, J., Tomás, J. M., & Benedi, V. J. (1999) · Microbiology · doi:10.1099/13500872-145-3-673 · Cited by 192 · see citing works
Naas, T., Vandel, L., Sougakoff, W., Livermore, D. M., & Nordmann, P. (1994) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.38.6.1262 · Cited by 184 · see citing works
Getting to the route of Helicobacter pylori treatment
Goddard, A. F. (1998) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/42.1.1 · Cited by 172 · see citing works
Role of primary structure and disulfide bond formation in beta-lactamase secretion
Pollitt, S. K., & Zalkin, H. (1983) · Journal of Bacteriology · doi:10.1128/jb.153.1.27-32.1983 · Cited by 171 · see citing works
Joris, B., Ledent, P., Dideberg, O., Fonzé, E., Lamotte‐Brasseur, J., Kelly, J. A., Ghuysen, J., & Frère, J. (1991) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.35.11.2294 · Cited by 167 · see citing works
Thompson, J. S., & Malamy, M. H. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.5.2584-2593.1990 · Cited by 165 · see citing works
Nordmann, P., Mariotte, S., Naas, T., Labia, R., & Nicolas-Chanoine, M.-H. (1993) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.37.5.939 · Cited by 161 · see citing works
Naas, T., & Nordmann, P. (1994) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.91.16.7693 · Cited by 159 · see citing works
Phylogeny of LCR‐1 and OXA‐5 with class A and class D β‐lactamases
Couture, F., Lachapelle, J., & Lévesque, R. C. (1992) · Molecular Microbiology · doi:10.1111/j.1365-2958.1992.tb00894.x · Cited by 149 · see citing works
Matthew, M., & HARRIS, A. M. (1976) · Journal of General Microbiology · doi:10.1099/00221287-94-1-55 · Cited by 142 · see citing works
Doménech‐Sánchez, A., Hernández-Allés, S., Martı́nez-Martı́nez, L., Benedi, V. J., & Albertí, S. (1999) · Journal of Bacteriology · doi:10.1128/jb.181.9.2726-2732.1999 · Cited by 140 · see citing works
Lee, E. H., Nicolas-Chanoine, M.-H., Kitzis, M.-D., Pialoux, G., Collatz, E., & Gutmann, L. (1991) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.35.6.1093 · Cited by 131 · see citing works
Stapleton, P. D., Shannon, K. P., & French, G. L. (1999) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.43.5.1206 · Cited by 128 · see citing works
Trends in β‐Lactam Resistance Among Enterobacteriaceae
Nordmann, P. (1998) · Clinical Infectious Diseases · doi:10.1086/514905 · Cited by 124 · see citing works
Charrel, R. N., Pagès, J., de Micco, P., & Malléa, M. (1996) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.40.12.2854 · Cited by 123 · see citing works
Walsh, T. R., MacGowan, A. P., & Bennett, P. M. (1997) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.41.7.1460 · Cited by 120 · see citing works
Ardanuy, C., Liñares, J., Domínguez, M. Á., et al. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.7.1636 · Cited by 98 · see citing works
McDougal, L. K., Tenover, F. C., Lee, L. N., et al. (1998) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.42.9.2312 · Cited by 94 · see citing works
Self-citationImipenem- and meropenem-resistant mutants of Enterobacter cloacae and Proteus rettgeri lack porins
Raimondi, A., Traverso, A., & Nikaido, H. (1991) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.35.6.1174 · Cited by 93 · see citing works
Swarén, P., Maveyraud, L., Raquet, X., Cabantous, S., Duez, C., Pédelacq, J. D., Mariotte-Boyer, S., Mourey, L., Labia, R., Nicolas-Chanoine, M.-H., et al. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.41.26714 · Cited by 85 · see citing works
Arakawa, Y., Ohta, M., Kido, N., et al. (1986) · FEBS Letters · doi:10.1016/0014-5793(86)80014-x · Cited by 81 · see citing works
Fournier, B., Roy, P. H., Lagrange, P. H., et al. (1996) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.40.2.454 · Cited by 76 · see citing works
Chow, J. W., & Shlaes, D. M. (1991) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/28.4.499 · Cited by 71 · see citing works
Mercier, J., & Lévesque, R. C. (1990) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.34.8.1577 · Cited by 65 · see citing works
Hopkins, J., & Towner, K. J. (1990) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/25.1.49 · Cited by 57 · see citing works
Mechanisms of resistance to beta-lactam antibiotics.
Dm, L. (1991) · PubMed · Cited by 55 · see citing works
Seoane, A., & Lobo, J. M. G. (1991) · Molecular and General Genetics MGG · doi:10.1007/bf00282468 · Cited by 49 · see citing works
Cloning and nucleotide sequencing of the gene encoding the β-lactamase from Citrobacter diversus
Perilli, M. (1991) · FEMS Microbiology Letters · doi:10.1016/0378-1097(91)90448-j · Cited by 48 · see citing works
de Champs, C., Henquell, C., Guélon, D., et al. (1993) · Journal of Clinical Microbiology · doi:10.1128/jcm.31.1.123-127.1993 · Cited by 47 · see citing works
Sequence of the PSE-1 β-Lactamase Gene
Huovinen, P., & Jacoby, G. A. (1991) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.35.11.2428 · Cited by 47 · see citing works
Villar, H. (1997) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/40.3.365 · Cited by 46 · see citing works
The Classification and Terminology of Enzymes That Hydrolyze -Lactam Antibiotics
SYKES, R. B. (1982) · The Journal of Infectious Diseases · doi:10.1093/infdis/145.2.762 · Cited by 46 · see citing works
Carbapenem resistance in a clinical isolate of Citrobacter freundii
Mainardi, J., Mugnier, P., Coutrot, A., et al. (1997) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.41.11.2352 · Cited by 41 · see citing works
Fournier, B., Gravel, A., Hooper, D. C., et al. (1999) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.43.4.850 · Cited by 40 · see citing works
Naas, T., Livermore, D. M., & Nordmann, P. (1995) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.39.3.629 · Cited by 39 · see citing works
Lachapelle, J. M., Dufresne, J., & Lévesque, R. C. (1991) · Gene · doi:10.1016/0378-1119(91)90530-o · Cited by 35 · see citing works
Structural and mechanistic aspects of evolution of beta-lactamases and penicillin-binding proteins.
Massova, I., & Mobashery, S. (1999) · PubMed · Cited by 35 · see citing works
Regulation of urease activity in the cyanobacterium Anabaena doliolum
Singh, S. (1990) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1990.tb13840.x · Cited by 34 · see citing works
A kinetic study of NMC-A β-lactamase, an Ambler class A carbapenemase also hydrolyzing cephamycins
Mariotte-Boyer, S., Nicolas, M. H., & Labia, R. (1996) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1996.tb08457.x · Cited by 34 · see citing works
Structural and Mechanistic Aspects of Evolution of β-Lactamases and Penicillin-Binding Proteins
Massova, I., & Mobashery, S. (1999) · Current Pharmaceutical Design · doi:10.2174/1381612805666230112193912 · Cited by 33 · see citing works
Cornaglia, G., Guan, L., Fontana, R., et al. (1992) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.36.9.1902 · Cited by 32 · see citing works
Bacterial Resistance to Carbapenems
Livermore, D. M. (1995) · Springer eBooks · doi:10.1007/978-1-4757-9203-4_3 · Cited by 26 · see citing works
Role of Ser-237 in the substrate specificity of the carbapenem-hydrolyzing class A β-lactamase Sme-1
Sougakoff, W., Naas, T., Nordmann, P., et al. (1999) · Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology · doi:10.1016/s0167-4838(99)00138-7 · Cited by 24 · see citing works
Ehrhardt, A. F., Sanders, C. C., Thomson, K. S., et al. (1993) · Clinical Infectious Diseases · doi:10.1093/clinids/17.1.120 · Cited by 22 · see citing works
Mechanisms responsible for reduced susceptibility to imipenem in Bacteroides fragilis
Edwards, R., & Greenwood, D. R. (1996) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/38.6.941 · Cited by 21 · see citing works
Prágai, Z., & Nagy, E. (1998) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/42.6.821 · Cited by 16 · see citing works
Saul, F. A., Normand, B. V.-L., Lema, F., & Bentley, G. A. (1997) · Proteins Structure Function and Bioinformatics · doi:10.1002/(sici)1097-0134(199701)27:1 · Cited by 13 · see citing works
Pm, H. (1997) · PubMed · Cited by 11 · see citing works
Winstanley, T. G. (1999) · Journal of Antimicrobial Chemotherapy · doi:10.1093/jac/43.6.850 · Cited by 9 · see citing works
Identification of a ROB-1 beta-lactamase in Haemophilus ducreyi
Maclean, I. W., Slaney, L., Juteau, J., et al. (1992) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.36.2.467 · Cited by 9 · see citing works
A kinetic study of NMC-A β-lactamase, an Ambler class A carbapenemase also hydrolyzing cephamycins
Mariotte-Boyer, S. (1996) · FEMS Microbiology Letters · doi:10.1016/0378-1097(96)00288-1 · Cited by 6 · see citing works
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Snitkin, E. S., Zelazny, A. M., Thomas, P. J., Stock, F., Program, N. C. S., Henderson, D. K., Palmore, T. N., & Segre, J. A. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3004129 · Cited by 863 · see citing works
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Wand, M. E., Bock, L. J., Bonney, L. C., & Sutton, J. M. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01162-16 · Cited by 313 · see citing works
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Aires, C. A. M., Pereira, P. S., Rocha-de-Souza, C. M., Silveira, M. C., Carvalho-Assef, A. P. D., & Asensi, M. D. (2020) · Microbial Drug Resistance · doi:10.1089/mdr.2019.0166 · Cited by 13 · see citing works
- Authors
- Yigit, H., Queenan, A. M., Anderson, G. J., Domenech-Sanchez, A., Biddle, J. W., Steward, C. D., Alberti, S., Bush, K., & Tenover, F. C.
- Affiliations
- Centers for Disease Control and Prevention (US)
- <!--label omitted: 2-->The R. W. Johnson Pharmaceutical Research Institute, Raritan, New Jersey 088692; and
- <!--label omitted: 3-->Área de Microbiologia, Universidad de las Islas Baleares, Crtra. Valldemosa, Palma de Mallorca, 07071,3 Spain
- <!--label omitted: 4-->Unidad de Investigacion, Hospital Son Dureta, Andrea Doria, Palma de Mallorca, 07014,4 and
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2001-04
- Volume
- 45
- Issue
- 4
- Pages
- 1151-1161
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC90438
- OpenAlex
- W2061729558
- Cited by
- 1,639
- References
- 75
- Citations by year
- 2026: 41 · 2025: 58 · 2024: 89 · 2023: 84 · 2022: 100 · 2021: 90 · 2020: 107 · 2019: 107 · 2018: 86 · 2017: 82 · 2016: 109 · 2015: 89 · 2014: 100 · 2013: 83 · 2012: 88
- FWCI
- 4.45
- Type
- journal article
- Language
- en
- Update notices
- correction (2008-02-01)
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Enterobacteriaceae and Cronobacter Research
- Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
- Keywords
- Klebsiella pneumoniae, carbapenemase, carbapenem resistance, beta-lactamase, imipenem, meropenem, extended-spectrum cephalosporins, aztreonam resistance, nonconjugative plasmids, plasmid-mediated gene transfer, clavulanic acid, tazobactam, outer membrane porins, OmpK35, OmpK36
- MeSH terms
- Meropenem, Amino Acid Sequence, Bacterial Outer Membrane Proteins, Base Sequence, beta-Lactamases, Cloning, Molecular, Escherichia coli, Kinetics, Klebsiella pneumoniae, Microbial Sensitivity Tests, Molecular Sequence Data, Phylogeny, Substrate Specificity, Thienamycins, Transformation, Bacterial, Imipenem, Carbapenems, Sequence Homology, Amino Acid, beta-Lactam Resistance
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