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Covalent labeling of protein components of the sodium channel with a photoactivable derivative of scorpion toxin.
Open accessAbstract
Azidonitrobenzoyl mono[125I]iodo scorpion toxin can be covalently attached to its receptor site in electrically excitable neuroblastoma cells or synaptosomes by photolysis. A polypeptide of Mr approximately 250,000 is specifically labeled in neuroblastoma cells. Labeling is blocked by unlabeled scorpion toxin and by depolarization. This polypeptide is not labeled in a variant neuroblastoma clone lacking voltage-sensitive sodium channels. Polypeptides of Mr approximately 250,000 and Mr approximately 32,000 are specifically labeled in synaptosomes. This labeling is also blocked by unlabeled toxin and by depolarization. These results identify specific polypeptides of Mr approximately 250,000 and Mr approximately 32,000 that are components of the sodium channel.
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Cited by
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Self-citationVoltage‐gated sodium channels at 60: structure, function and pathophysiology
Catterall, W. A. (2012) · The Journal of Physiology · doi:10.1113/jphysiol.2011.224204 · Cited by 719
Self-citationVoltage-gated sodium channels: structure, function, pharmacology, and clinical indications
de Lera Ruiz, M., & Kraus, R. L. (2015) · Journal of Medicinal Chemistry · doi:10.1021/jm501981g · Cited by 548
The sodium channel from rat brain. Purification and subunit composition.
Hartshorne, R. P., & Catterall, W. A. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)43460-0 · Cited by 356
Self-citationCestèle, S., Qu, Y., Rogers, J. C., Rochat, H., Scheuer, T., & Catterall, W. A. (1998) · Neuron · doi:10.1016/s0896-6273(00)80606-6 · Cited by 335
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Metadata
- Authors
- Beneski, D. A., & Catterall, W. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1980-01
- Volume
- 77
- Issue
- 1
- Pages
- 639-643
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2056464913
- Cited by
- 203
- References
- 0
- FWCI
- 5.93
- Type
- journal article
- Language
- en
- Open access
- green
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- Beneski, D. A., & Catterall, W. A. (1980). Covalent labeling of protein components of the sodium channel with a photoactivable derivative of scorpion toxin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.77.1.639
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