The DOI matches its Crossref record.
Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
Open accessAbstract
The yeast Saccharomyces cerevisiae is known to contain the highly conserved and unbiquitous protein actin. We have used cloned actin sequences from Dictyostelium discoideum to identify and clone the actin gene in yeast. Hybridization to genomic fragments of yeast DNA suggest that there is a single actin gene in yeast. We have determined the nucleotide sequence of that gene and its flanking regions. The sequence of the gene reveals an intervening sequence of 309 base pairs in the coding sequences at the 5' end of the gene. The existence and location of the intervening sequence was verified by using the dideoxy chain termination technique to determine the sequence at the 5' terminus of the actin mRNA. The similarity of the splice junction sequences in this gene to those found in higher eukaryotes suggests that yeast must possess a similar splicing enzyme.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
17 beta-estradiol hydroxylation catalyzed by human cytochrome P450 1B1.
Hayes, C. L., Spink, D. C., Spink, B. C., Cao, J. Q., Walker, N. J., & Sutter, T. R. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.18.9776 · Cited by 632
Berger, S. L., Piña, B., Silverman, N., Marcus, G. A., Agapite, J., Regier, J. L., Triezenberg, S. J., & Guarente, L. (1992) · Cell · doi:10.1016/0092-8674(92)90100-q · Cited by 435
Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae.
Vijayraghavan, U., Company, M., & Abelson, J. (1989) · Genes & Development · doi:10.1101/gad.3.8.1206 · Cited by 331
Self-citationSystematic mutational analysis of the yeast ACT1 gene.
Wertman, K. F., Drubin, D. G., & Botstein, D. (1992) · Genetics · doi:10.1093/genetics/132.2.337 · Cited by 314
Two distinct classes of keratin genes and their evolutionary significance
FUCHS, E. (1981) · Cell · doi:10.1016/0092-8674(81)90362-7 · Cited by 305
Metadata
- Authors
- Ng, R., & Abelson, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1980-07
- Volume
- 77
- Issue
- 7
- Pages
- 3912-3916
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2081090611
- Cited by
- 546
- References
- 0
- FWCI
- 14.81
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Isolation and sequence of the gene for actin in Saccharomyces cerevisiae. https://app.cochat.ai/papers/10.1073/pnas.77.7.3912
- The paper
- Ng, R., & Abelson, J. (1980). Isolation and sequence of the gene for actin in Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.77.7.3912
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
