The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
17 beta-estradiol hydroxylation catalyzed by human cytochrome P450 1B1.
Open accessAbstract
The 4-hydroxy metabolite of 17 beta-estradiol (E2) has been implicated in the carcinogenicity of this hormone. Previous studies showed that aryl hydrocarbon-receptor agonists induced a cytochrome P450 that catalyzed the 4-hydroxylation of E2. This activity was associated with human P450 1B1. To determine the relationship of the human P450 1B1 gene product and E2 4-hydroxylation, the protein was expressed in Saccharomyces cerevisiae. Microsomes from the transformed yeast catalyzed the 4- and 2-hydroxylation of E2 with Km values of 0.71 and 0.78 microM and turnover numbers of 1.39 and 0.27 nmol product min-1.nmol P450-1, respectively. Treatment of MCF-7 human breast cancer cells with the aryl hydrocarbon-receptor ligand indolo[3,2-b]carbazole resulted in a concentration-dependent increase in P450 1B1 and P450 1A1 mRNA levels, and caused increased rates of 2-, 4-, 6 alpha-, and 15 alpha-hydroxylation of E2. At an E2 concentration of 10 nM, the increased rates of 2- and 4-hydroxylation were approximately equal, emphasizing the significance of the low Km P450 1B1-component of E2 metabolism. These studies demonstrate that human P450 1B1 is a catalytically efficient E2 4-hydroxylase that is likely to participate in endocrine regulation and the toxicity of estrogens.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Activation of chemically diverse procarcinogens by human cytochrome P-450 1B1.
Shimada, T., Hayes, C. L., Yamazaki, H., et al. (1996) · PubMed · Cited by 793
Self-citationBjeldanes, L. F., Kim, J. Y., Grose, K. R., Bartholomew, J. C., & Bradfield, C. A. (1991) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.88.21.9543 · Cited by 588
Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.
Ng, R., & Abelson, J. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.7.3912 · Cited by 546
Sutter, T. R., Tang, Y. M., Hayes, C. L., Wo, Y. Y., Jabs, E. W., Li, X., Yin, H., Cody, C. W., & Greenlee, W. F. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36803-5 · Cited by 508
Self-citationde Waziers, I., Cugnenc, P. H., Yang, C. S., Leroux, J. P., & Beaune, P. H. (1990) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)12474-9 · Cited by 502
4-Hydroxylation of estrogens as marker of human mammary tumors.
Liehr, J. G., & Ricci, M. J. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.8.3294 · Cited by 414
Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae
Dobson, M. J., Tuite, M. F., Roberts, N. A., Kingsman, A. J., Kingsman, S. M., Perkins, R. E., Conroy, S. C., Dunbar, B., & Fothergill, L. A. (1982) · Nucleic Acids Research · doi:10.1093/nar/10.8.2625 · Cited by 405
Carcinogenicity of catechol estrogens in Syrian hamsters
Liehr, J. G., Wan-Fen, F., Sirbasku, D. A., & Ari-Ulubelen, A. (1986) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(86)90080-4 · Cited by 356
Toward the Primary Prevention of Cancer
Henderson, B. E., Ross, R. K., & Pike, M. C. (1991) · Science · doi:10.1126/science.1957166 · Cited by 355
Targets for Dioxin: Genes for Plasminogen Activator Inhibitor-2 and Interleukin-1β
Sutter, T. R., Guzman, K., Dold, K. M., & Greenlee, W. F. (1991) · Science · doi:10.1126/science.1925598 · Cited by 337
Self-citation
Cited by
Männistö, P. T., & Kaakkola, S. (1999) · Pharmacological Reviews · doi:10.1016/s0031-6997(24)01423-6 · Cited by 1,068
Cytochrome P450-mediated metabolism of estrogens and its regulation in human
Tsuchiya, Y., Nakajima, M., & Yokoi, T. (2005) · Cancer Letters · doi:10.1016/j.canlet.2004.10.007 · Cited by 616
Shanle, E. K., & Xu, W. (2011) · Chemical Research in Toxicology · doi:10.1021/tx100231n · Cited by 522
Schiffer, L., Barnard, L., Baranowski, E. S., Gilligan, L. C., Taylor, A. E., Arlt, W., Shackleton, C. H. L., & Storbeck, K.-H. (2019) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2019.105439 · Cited by 450
Frye, C., Bo, E., Calamandrei, G., Calzà, L., Dessì‐Fulgheri, F., Fernández, M., Fusani, L., Kah, O., Kajta, M., Le Page, Y., et al. (2012) · Journal of Neuroendocrinology · doi:10.1111/j.1365-2826.2011.02229.x · Cited by 431
Metadata
- Authors
- Hayes, C. L., Spink, D. C., Spink, B. C., Cao, J. Q., Walker, N. J., & Sutter, T. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1996-09-03
- Volume
- 93
- Issue
- 18
- Pages
- 9776-9781
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2052528439
- Cited by
- 632
- References
- 44
- FWCI
- 19.11
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: 17 beta-estradiol hydroxylation catalyzed by human cytochrome P450 1B1. https://app.cochat.ai/papers/10.1073/pnas.93.18.9776
- The paper
- Hayes, C. L., Spink, D. C., Spink, B. C., Cao, J. Q., Walker, N. J., & Sutter, T. R. (1996). 17 beta-estradiol hydroxylation catalyzed by human cytochrome P450 1B1. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.93.18.9776
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
