The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Systematic mutational analysis of the yeast ACT1 gene.
Open accessAbstract
We report the isolation and characterization of a synoptic set of site-directed mutations distributed throughout the single actin gene of Saccharomyces cerevisiae. Mutations were systematically targeted to the surface of the protein by identifying clusters of 2 or more charged residues in the primary sequence; every charged residue in a cluster was replaced with alanine. Mutations were recovered in high yield (34 of 36 constructed) as heterozygous diploids. Mutant phenotypes were examined in haploid segregants: 11 were recessive lethal, 16 conditional-lethal (including temperature-sensitive and salt-sensitive) and 7 had no discernible phenotype. Genetic analysis suggested that the two mutations constructed but not recovered in yeast may have a dominant defective phenotype. Location of the mutant residues on the three-dimensional structure of the rabbit muscle actin monomer confirmed that most (81%) of the charged residues we altered lie at or near the surface of the protein, confirming a key assumption of the method. Many of the new act1 alleles have properties readily interpreted in light of the actin structure and should prove useful in both genetic and biochemical studies of actin function.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,249
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Transformation of intact yeast cells treated with alkali cations
Ito, H., Fukuda, Y., Murata, K., & Kimura, A. (1983) · Journal of Bacteriology · doi:10.1128/jb.153.1.163-168.1983 · Cited by 7,518
Cold Spring Harbor Laboratory; Cold Spring Harbor, NY: 1979 xxxvi + 1388 pages. $120.00
Cavalier-Smith, T. (1980) · FEBS Letters · doi:10.1016/0014-5793(80)80323-1 · Cited by 6,273
[19] Rapid and efficient site-specific mutagenesis without phenotypic selection
Kunkel, T. A., Roberts, J. D., & Zakour, R. A. (1987) · Methods in Enzymology · doi:10.1016/0076-6879(87)54085-x · Cited by 5,261
[1] Production of single-stranded plasmid DNA
Vieira, J., & Messing, J. (1987) · Methods in Enzymology · doi:10.1016/0076-6879(87)53044-0 · Cited by 2,597
Hoffman, C. S., & Winston, F. (1987) · Gene · doi:10.1016/0378-1119(87)90131-4 · Cited by 2,479
Atomic structure of the actin: DNase I complex
Kabsch, W., Mannherz, H. G., Suck, D., Pai, E. F., & Holmes, K. C. (1990) · Nature · doi:10.1038/347037a0 · Cited by 1,928
Atomic model of the actin filament
Holmes, K. C., Popp, D., Gebhard, W., & Kabsch, W. (1990) · Nature · doi:10.1038/347044a0 · Cited by 1,697
Cited by
Osmotic Stress Signaling and Osmoadaptation in Yeasts
Hohmann, S. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.2.300-372.2002 · Cited by 1,767
Wimmer, E., Hellen, C. U. T., & Cao, X. (1993) · Annual Review of Genetics · doi:10.1146/annurev.ge.27.120193.002033 · Cited by 554
Latrunculin alters the actin-monomer subunit interface to prevent polymerization
Morton, W. M., Ayscough, K. R., & McLaughlin, P. J. (2000) · Nature Cell Biology · doi:10.1038/35014075 · Cited by 523
Reggiori, F., Monastyrska, I., Shintani, T., & Klionsky, D. J. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e05-07-0629 · Cited by 155
The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast.
Wang, T., & Bretscher, A. (1995) · Molecular Biology of the Cell · doi:10.1091/mbc.6.8.1011 · Cited by 64
Metadata
- Authors
- Wertman, K. F., Drubin, D. G., & Botstein, D.
- Venue
- Genetics
- Publisher
- Oxford University Press (OUP)
- Published
- 1992-10-01
- Volume
- 132
- Issue
- 2
- Pages
- 337-350
- ISSN
- 0016-6731 (ISSN-L)
- 1943-2631
- OpenAlex
- W1875421809
- Cited by
- 314
- References
- 33
- FWCI
- 6.23
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Systematic mutational analysis of the yeast ACT1 gene. https://app.cochat.ai/papers/10.1093/genetics/132.2.337
- The paper
- Wertman, K. F., Drubin, D. G., & Botstein, D. (1992). Systematic mutational analysis of the yeast ACT1 gene. Genetics. https://doi.org/10.1093/genetics/132.2.337
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
