The DOI matches its Crossref record.
Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome.
Open accessAbstract
The complete 7410 nucleotide sequence of poliovirus type I genome was obtained from cloned cDNA. Double-stranded poliovirus cDNA was synthesized and inserted into the Pst I site of plasmid pBR322, and three clones were derived that together provided DNA copies of the entire poliovirus genome. Two of the clones contained inserts of 2.5 and 6.5 kilobases and represented all but the 5' 115 bases of poliovirus RNA. A third clone was generated from primer-extended DNA and contained sequences from the 5' end of the viral RNA. An open reading frame that was identified in the nucleotide sequence starting 743 bases from the 5' end of the RNA and extending to a termination codon 71 bases from the 3' end contained known poliovirus polypeptide sequence.
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Citations
Cited by
Cello, J., Paul, A. V., & Wimmer, E. (2002) · Science · doi:10.1126/science.1072266 · Cited by 980
VACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION
Kew, O. M., Sutter, R. W., de Gourville, E. M., Dowdle, W. R., & Pallansch, M. A. (2005) · Annual Review of Microbiology · doi:10.1146/annurev.micro.58.030603.123625 · Cited by 703
Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells
Racaniello, V. R., & Baltimore, D. (1981) · Science · doi:10.1126/science.6272391 · Cited by 696
Self-citationA functional ribonucleoprotein complex forms around the 5′ end of poliovirus RNA
Andino, R., Rieckhof, G. E., & Baltimore, D. (1990) · Cell · doi:10.1016/0092-8674(90)90170-j · Cited by 494
Self-citationThe life cycle of non-polio enteroviruses and how to target it
Baggen, J., Thibaut, H. J., Strating, J. R. P. M., & van Kuppeveld, F. J. M. (2018) · Nature Reviews Microbiology · doi:10.1038/s41579-018-0005-4 · Cited by 478
Metadata
- Authors
- Racaniello, V. R., & Baltimore, D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1981-08
- Volume
- 78
- Issue
- 8
- Pages
- 4887-4891
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2157707773
- Cited by
- 550
- References
- 0
- FWCI
- 28.26
- Type
- journal article
- Language
- en
- Open access
- green
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- Racaniello, V. R., & Baltimore, D. (1981). Molecular cloning of poliovirus cDNA and determination of the complete nucleotide sequence of the viral genome. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.78.8.4887
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