Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells
Abstract
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Only two nucleotide substitutions (G481A in the IRES, and C2909U encoding a T → I substitution at position 143 of VP1) appear to be responsible for the attenuated phenotype of Sabin 2.
“Only two nucleotide substitutions (G481A in the IRES, and C2909U encoding a T → I substitution at position 143 of VP1) appear to be responsible for the attenuated phenotype of Sabin 2 (111, 160)”
From VACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION (2005) · cites this paper as [160]
Cites
Preparative and analytical purification of DNA from agarose.
Vogelstein, B., & Gillespie, D. A. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.2.615 · Cited by 1,555 · see citing works
Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells
Dagert, M., & Ehrlich, S. D. (1979) · Gene · doi:10.1016/0378-1119(79)90082-9 · Cited by 1,417 · see citing works
Expression of animal virus genomes
Baltimore, D. (1971) · Bacteriological Reviews · doi:10.1128/br.35.3.235-241.1971 · Cited by 918 · see citing works
Self-citationPrimary structure, gene organization and polypeptide expression of poliovirus RNA
Kitamura, N., Semler, B. L., Rothberg, P. G., Larsen, G. R., Adler, C. J., Dorner, A. J., Emini, E. A., Hanecak, R., Lee, J. J., van der Werf, S., et al. (1981) · Nature · doi:10.1038/291547a0 · Cited by 914 · see citing works
Bothwell, A. L. M., Paskind, M. P., Reth, M. G., et al. (1981) · Cell · doi:10.1016/0092-8674(81)90089-1 · Cited by 674 · see citing works
Parker, B. A., & Stark, G. R. (1979) · Journal of Virology · doi:10.1128/jvi.31.2.360-369.1979 · Cited by 622 · see citing works
Racaniello, V. R., & Baltimore, D. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.8.4887 · Cited by 555 · see citing works
Self-citationQβ DNA-containing hybrid plasmids giving rise to Qβ phage formation in the bacterial host
Taniguchi, T., Palmieri, M., & Weissmann, C. (1978) · Nature · doi:10.1038/274223a0 · Cited by 203 · see citing works
Molecular Weight of Poliovirus Ribonucleic Acid
Granboulan, N., & Girard, M. P. (1969) · Journal of Virology · doi:10.1128/jvi.4.4.475-479.1969 · Cited by 108 · see citing works
Evidence for the nonparticipation of DNA in viral RNA synthesis
Simon, E. H. (1961) · Virology · doi:10.1016/0042-6822(61)90037-x · Cited by 102 · see citing works
Hewlett, M. J., & Florkiewicz, R. Z. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.1.303 · Cited by 33 · see citing works
Nomoto, A., Toyoda, H., & Imura, N. (1981) · Virology · doi:10.1016/0042-6822(81)90135-5 · Cited by 13 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
mRNA-based therapeutics--developing a new class of drugs
Sahin, U., Karikó, K., & Türeci, Ö. (2014) · Nature Reviews Drug Discovery · doi:10.1038/nrd4278 · Cited by 2,474 · see citing works
VACCINE-DERIVED POLIOVIRUSES AND THE ENDGAME STRATEGY FOR GLOBAL POLIO ERADICATION
Kew, O. M., Sutter, R. W., de Gourville, E. M., Dowdle, W. R., & Pallansch, M. A. (2005) · Annual Review of Microbiology · doi:10.1146/annurev.micro.58.030603.123625 · Cited by 703 · see citing works
Wimmer, E., Hellen, C. U. T., & Cao, X. (1993) · Annual Review of Genetics · doi:10.1146/annurev.ge.27.120193.002033 · Cited by 554 · see citing works
Kawamura, N., Kohara, M., Abe, S., Komatsu, T., Tago, K., Arita, M., & Nomoto, A. (1989) · Journal of Virology · doi:10.1128/jvi.63.3.1302-1309.1989 · Cited by 232 · see citing works
PLA2G16 represents a switch between entry and clearance of Picornaviridae
Staring, J., von Castelmur, E., Blomen, V. A., van den Hengel, L. G., Brockmann, M., Baggen, J., Thibaut, H. J., Nieuwenhuis, J., Janssen, H., van Kuppeveld, F. J. M., et al. (2017) · Nature · doi:10.1038/nature21032 · Cited by 201 · see citing works
Genetic analysis of the attenuation phenotype of poliovirus type 1
Omata, T., Kohara, M., Kuge, S., Komatsu, T., Abe, S., Semler, B. L., Kameda, A., Itoh, H., Arita, M., & Wimmer, E. (1986) · Journal of Virology · doi:10.1128/jvi.58.2.348-358.1986 · Cited by 190 · see citing works
Stanway, G., Hughes, P. J., Mountford, R. C., Reeve, P., Minor, P. D., Schild, G. C., & Almond, J. W. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.5.1539 · Cited by 188 · see citing works
Determinants of attenuation and temperature sensitivity in the type 1 poliovirus Sabin vaccine
Bouchard, M. J., Lam, D. H., & Racaniello, V. R. (1995) · Journal of Virology · doi:10.1128/jvi.69.8.4972-4978.1995 · Cited by 140 · see citing works
Self-citationPoliovirus and poliomyelitis: A tale of guts, brains, and an accidental event
Mueller, S., Wimmer, E., & Cello, J. (2005) · Virus Research · doi:10.1016/j.virusres.2005.04.008 · Cited by 133 · see citing works
Cloning and synthesis of infectious cardiovirus RNAs containing short, discrete poly(C) tracts
Duke, G. M., & Palmenberg, A. C. (1989) · Journal of Virology · doi:10.1128/jvi.63.4.1822-1826.1989 · Cited by 120 · see citing works
Svitkin, Y. V., Cammack, N., Minor, P. D., & Almond, J. W. (1990) · Virology · doi:10.1016/0042-6822(90)90190-3 · Cited by 116 · see citing works
Iizuka, N., Kohara, M., Hagino-Yamagishi, K., Abe, S., Komatsu, T., Tago, K., Arita, M., & Nomoto, A. (1989) · Journal of Virology · doi:10.1128/jvi.63.12.5354-5363.1989 · Cited by 107 · see citing works
Minor, P. D., Dunn, G., Evans, D. M. A., Magrath, D. I., John, A., Howlett, J., Phillips, A., Westrop, G., Wareham, K., Almond, J. W., et al. (1989) · Journal of General Virology · doi:10.1099/0022-1317-70-5-1117 · Cited by 82 · see citing works
Morrison, J. M., & Racaniello, V. R. (2009) · Journal of Virology · doi:10.1128/jvi.02177-08 · Cited by 51 · see citing works
Self-citationStrategy for construction of live picornavirus vaccines
Nomoto, A., Iizuka, N., Kohara, M., & Arita, M. (1988) · Vaccine · doi:10.1016/s0264-410x(88)80015-x · Cited by 24 · see citing works
- Authors
- Racaniello, V. R., & Baltimore, D.
- Affiliations
- Massachusetts Institute of Technology (US)
- Venue
- Science
- Published
- 1981-11-20
- Volume
- 214
- Issue
- 4523
- Pages
- 916-919
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2041934325
- Cited by
- 696
- References
- 12
- Citations by year
- 2026: 7 · 2025: 6 · 2024: 13 · 2023: 10 · 2022: 14 · 2021: 11 · 2020: 20 · 2019: 14 · 2018: 10 · 2017: 13 · 2016: 14 · 2015: 10 · 2014: 23 · 2013: 11 · 2012: 16
- FWCI
- 19.21
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
- Keywords
- poliovirus, cDNA, infectious virus production, transfection, mammalian cells, viral RNA genome, plasmid cloning
- MeSH terms
- Animals, Cloning, Molecular, DNA Restriction Enzymes, DNA, Recombinant, DNA, Viral, Escherichia coli, Genes, Viral, Plasmids, RNA, Viral, Transfection, Poliovirus
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review