Cited by 696Viral Infections and Immunology ResearchVirology and Viral DiseasesAnimal Virus Infections Studies

Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells

Racaniello, Vincent R., Baltimore, David·1981·Science

Abstract

A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.

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Registry

Crossref · 10.1126/science.6272391

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12 of 12 references verified0 citation errors · 0 not checkable

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  • Only two nucleotide substitutions (G481A in the IRES, and C2909U encoding a T → I substitution at position 143 of VP1) appear to be responsible for the attenuated phenotype of Sabin 2.

    “Only two nucleotide substitutions (G481A in the IRES, and C2909U encoding a T → I substitution at position 143 of VP1) appear to be responsible for the attenuated phenotype of Sabin 2 (111, 160)”

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Authors
Racaniello, V. R., & Baltimore, D.
Affiliations
Massachusetts Institute of Technology (US)
Venue
Science
Published
1981-11-20
Volume
214
Issue
4523
Pages
916-919
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2041934325
Cited by
696
References
12
Citations by year
2026: 7 · 2025: 6 · 2024: 13 · 2023: 10 · 2022: 14 · 2021: 11 · 2020: 20 · 2019: 14 · 2018: 10 · 2017: 13 · 2016: 14 · 2015: 10 · 2014: 23 · 2013: 11 · 2012: 16
FWCI
19.21
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
  • Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Animal Virus Infections Studies
Keywords
poliovirus, cDNA, infectious virus production, transfection, mammalian cells, viral RNA genome, plasmid cloning
MeSH terms
Animals, Cloning, Molecular, DNA Restriction Enzymes, DNA, Recombinant, DNA, Viral, Escherichia coli, Genes, Viral, Plasmids, RNA, Viral, Transfection, Poliovirus

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