Chemical synthesis of poliovirus cDNA: generation of infectious virus in the absence of natural template.
Abstract
Full-length poliovirus complementary DNA (cDNA) was synthesized by assembling oligonucleotides of plus and minus strand polarity. The synthetic poliovirus cDNA was transcribed by RNA polymerase into viral RNA, which translated and replicated in a cell-free extract, resulting in the de novo synthesis of infectious poliovirus. Experiments in tissue culture using neutralizing antibodies and CD155 receptor-specific antibodies and neurovirulence tests in CD155 transgenic mice confirmed that the synthetic virus had biochemical and pathogenic characteristics of poliovirus. Our results show that it is possible to synthesize an infectious agent by in vitro chemical-biochemical means solely by following instructions from a written sequence.
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In early studies of de novo genome synthesis, route B (PCR amplification using primers produced by traditional column-based oligo synthesizer) was preferred.
“In early studies of de novo genome synthesis, route B is preferred”
From Large-Scale de Novo Oligonucleotide Synthesis for Whole-Genome Synthesis and Data Storage: Challenges and Opportunities (2021) · cites this paper as Cello et al., 2002
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- Authors
- Cello, J., Paul, A. V., & Wimmer, E.
- Affiliations
- Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794–5222, USA.
- State University of New York (US)
- Corresponding author
- Wimmer, Eckard
- ORCID
- Wimmer, Eckard
- Venue
- Science
- Published
- 2002-08-09
- Volume
- 297
- Issue
- 5583
- Pages
- 1016-1018
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2043170430
- Cited by
- 980
- References
- 21
- Citations by year
- 2026: 27 · 2025: 29 · 2024: 44 · 2023: 30 · 2022: 33 · 2021: 67 · 2020: 39 · 2019: 33 · 2018: 36 · 2017: 34 · 2016: 38 · 2015: 41 · 2014: 50 · 2013: 40 · 2012: 44
- FWCI
- 18.42
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Viral Infections and Immunology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Health Sciences › Medicine › Epidemiology › Virology and Viral Diseases
- Keywords
- poliovirus, oligonucleotide assembly, neurovirulence, transgenic mice, viral RNA transcription, pathogenicity
- MeSH terms
- Animals, Antibodies, Monoclonal, Capsid, Cell-Free System, Female, HeLa Cells, Humans, Male, Membrane Proteins, Mice, Transgenic, Neutralization Tests, Viral Plaque Assay, Poliomyelitis, Promoter Regions, Genetic, Receptors, Virus, DNA-Directed RNA Polymerases, RNA, Viral, Transcription, Genetic, Protein Biosynthesis, Viral Proteins, Virulence, Virus Replication, Genome, Viral, Poliovirus, DNA, Complementary, Mice
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