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Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes.
Open accessAbstract
Coprecipitation of DNA with calcium phosphate is a commonly used method of gene transfer in mammalian cells. We have found that DNA forms a tight complex with Ca Pi and that DNA in this complex is resistant to nucleases present in serum or added externally. Under optimal conditions, virtually all of the recipient mouse Ltk- Aprt- cells take up Ca Pi--DNA complexes, as determined by fluorescent dyes specific for DNA (4',6-diaminilo-2-phenylindol dihydrochloride) or for calcium salts (chlorotetracycline). However, only a small proportion of the cells have detectable CA Pi--DNA complexes in the nucleus. Uptake of the Ca Pi--DNA complexes was highly dependent upon the pH at which the Ca Pi--DNA complexes was formed and upon the concentration of DNA in the complex.
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Citations
Cited by
Sodroski, J. G., Rosen, C. A., & Haseltine, W. A. (1984) · Science · doi:10.1126/science.6330891 · Cited by 770
New procedure for DNA transfection with polycation and dimethyl sulfoxide.
Kawai, S., & Nishizawa, M. (1984) · Molecular and Cellular Biology · doi:10.1128/mcb.4.6.1172 · Cited by 494
Large-Scale transfection of mammalian cells for the fast production of recombinant protein
Pham, P. L., Kamen, A., & Durocher, Y. (2006) · Molecular Biotechnology · doi:10.1385/mb:34:2:225 · Cited by 236
Bansal, G. S., & Latchman, D. S. (1990) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(90)90080-c · Cited by 4
Metadata
- Authors
- Loyter, A., Scangos, G. A., & Ruddle, F. H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1982-01
- Volume
- 79
- Issue
- 2
- Pages
- 422-426
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1973430331
- Cited by
- 220
- References
- 0
- FWCI
- 8.18
- Type
- journal article
- Language
- en
- Open access
- green
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- Loyter, A., Scangos, G. A., & Ruddle, F. H. (1982). Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.79.2.422
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
