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Cited by 494

The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.

New procedure for DNA transfection with polycation and dimethyl sulfoxide.

Open access
Kawai, S, Nishizawa, M·1984·Molecular and Cellular Biology

Abstract

A new procedure for DNA transfection has been developed in a system of chicken embryo fibroblast cells and cloned Rous sarcoma virus DNA by using a polycation reagent as a mediator to adsorb DNA to the cell surface and dimethyl sulfoxide as an agent to facilitate the uptake of adsorbed DNA by the cells. In this new, simple, and convenient polycation-dimethyl sulfoxide transfection, which requires no carrier DNA even with small amounts of DNA, the number of transformed cell foci induced by Rous sarcoma virus DNA was proportional to the dose of the transfecting DNA, and chicken embryo fibroblast cells were successfully transformed by v-src-containing subgenomic DNA as well.

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Crossref · 10.1128/mcb.4.6.1172

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Metadata

Authors
Kawai, S., & Nishizawa, M.
Venue
Molecular and Cellular Biology
Published
1984-06
Volume
4
Issue
6
Pages
1172-1174
ISSN
  • 0270-7306 (ISSN-L)
  • 1098-5549
OpenAlex
W1833064266
Cited by
494
References
12
FWCI
6.03
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: New procedure for DNA transfection with polycation and dimethyl sulfoxide. https://app.cochat.ai/papers/10.1128/mcb.4.6.1172
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Kawai, S., & Nishizawa, M. (1984). New procedure for DNA transfection with polycation and dimethyl sulfoxide. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.4.6.1172

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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.