The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Activation of purified soluble guanylate cyclase by protoporphyrin IX.
Open accessAbstract
Soluble guanylate cyclase [GTP pyrophosphate-lyase (cyclizing), EC 4.6.1.2] purified from bovine lung is markedly activated (30- to 40-fold) by protoporphyrin IX (Ka, 15-25 nM) and is inhibited by hematin (Ki, 3.7 microM) when MgGTP is used as substrate. Guanylate cyclase possesses specific activities (mumol of cGMP per min/mg of protein) of 0.1-0.2 (MgGTP) and 0.3-0.5 (MnGTP) and can attain values of 2-8 (MgGTP) or 1-1.4 (MnGTP) in the presence of protoporphyrin IX. Guanylate cyclase purified in this study contains heme and is activated by nitric oxide and nitrosyl-heme to the same magnitude as that by protoporphyrin IX. With the exception of hematoporphyrin IX, close structural analogs of protoporphyrin IX, including precursors and metabolites, do not activate guanylate cyclase. The insertion of iron into protoporphyrin IX to form heme or hematin renders the metalloporphyrin an inhibitor of unactivated or activated guanylate cyclase. The data suggest that protoporphyrin IX and heme could function to modulate guanylate cyclase activity.
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Cites
Ignarro, L. J., Lippton, H., Edwards, J. C., Baricos, W. H., Hyman, A. L., Kadowitz, P. J., & Gruetter, C. A. (1981) · The Journal of Pharmacology and Experimental Therapeutics · doi:10.1016/s0022-3565(25)32738-2 · Cited by 1,633
Self-citation
Cited by
Biosynthesis and Metabolism of Endothelium-Derived Nitric Oxide
Ignarro, L. J. (1990) · Annual Review of Pharmacology and Toxicology · doi:10.1146/annurev.pa.30.040190.002535 · Cited by 1,314
Self-citationIgnarro, L. J., Fukuto, J. M., Griscavage, J. M., Rogers, N. E., & Byrns, R. E. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.17.8103 · Cited by 856
Regulation of Nitric Oxide-Sensitive Guanylyl Cyclase
Friebe, A., & Koesling, D. (2003) · Circulation Research · doi:10.1161/01.res.0000082524.34487.31 · Cited by 508
Structure and Regulation of Soluble Guanylate Cyclase
Derbyshire, E. R., & Marletta, M. A. (2012) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-050410-100030 · Cited by 466
A molecular basis for nitric oxide sensing by soluble guanylate cyclase
Zhao, Y., Brandish, P. E., Ballou, D. P., & Marletta, M. A. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.26.14753 · Cited by 323
Metadata
- Authors
- Ignarro, L. J., Wood, K. S., & Wolin, M. S.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1982-05
- Volume
- 79
- Issue
- 9
- Pages
- 2870-2873
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1991694168
- Cited by
- 207
- References
- 1
- FWCI
- 5.19
- Type
- journal article
- Language
- en
- Open access
- green
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- Ignarro, L. J., Wood, K. S., & Wolin, M. S. (1982). Activation of purified soluble guanylate cyclase by protoporphyrin IX. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.79.9.2870
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