The DOI matches its Crossref record; 21 of the 21 references it cites were found in a registry or an index.
Methyl-directed repair of DNA base-pair mismatches in vitro.
Open accessAbstract
An assay has been developed that permits analysis of DNA mismatch repair in cell-free extracts of Escherichia coli. The method relies on repair of heteroduplex molecules of f1 R229 DNA, which contain a base-pair mismatch within the single EcoRI site of the molecule. As observed with mismatch heteroduplexes of lambda DNA [Pukkila, P. J., Peterson, J., Herman, G., Modrich, P. & Meselson, M. (1983) Genetics, in press], in vivo mismatch correction of f1 heteroduplexes is directed by the state of dam methylation of d(G-A-T-C) sequences within the DNA duplex. Thus, the heteroduplex (formula: see book) is repaired in vivo to an EcoRI-sensitive form if the strand bearing the wild-type EcoRI sequence carries the dam modification and the other does not. Such molecules are also subject to mismatch repair by E. coli extracts. The in vitro activity is also dependent on ATP, the state of dam methylation of mismatch heteroduplexes, and products of mutH, mutL, mutS, and uvrE loci. However, crude fractions deficient in these gene products do complement in the cell-free system, thus providing assays for their isolation. The in vitro reaction is accompanied by repair synthesis on the unmethylated DNA strand.
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Citations
Cites
Experiments in molecular genetics
Miller, J. H. (1972) · Medical Entomology and Zoology · Cited by 27,157
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
[57] Sequencing end-labeled DNA with base-specific chemical cleavages
Maxam, A. M., & Gilbert, W. (1980) · Methods in Enzymology · doi:10.1016/s0076-6879(80)65059-9 · Cited by 14,315
[29] Analysis of repeating DNA sequences by reassociation
Britten, R. J., Graham, D. E., & Neufeld, B. R. (1974) · Methods in Enzymology · doi:10.1016/0076-6879(74)29033-5 · Cited by 1,033
DNA N-glycosidases: properties of uracil-DNA glycosidase from Escherichia coli.
Lindahl, T., Ljungquist, S., Siegert, W., Nyberg, B., & Sperens, B. (1977) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)40386-3 · Cited by 522
Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.
Glickman, B. W., & Radman, M. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.2.1063 · Cited by 395
BACTERIAL MUTATOR GENES AND THE CONTROL OF SPONTANEOUS MUTATION
Cox, E. C. (1976) · Annual Review of Genetics · doi:10.1146/annurev.ge.10.120176.001031 · Cited by 340
Repair tracts in mismatched DNA heteroduplexes.
Wagner, R., & Meselson, M. (1976) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.73.11.4135 · Cited by 301
RNA Synthesis Initiates In Vitro Conversion of M13 DNA to Its Replicative Form
Wickner, W., Brutlag, D., Schekman, R., & Kornberg, A. (1972) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.69.4.965 · Cited by 300
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Cited by
Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli
Walker, G. C. (1984) · Microbiological Reviews · doi:10.1128/mmbr.48.1.60-93.1984 · Cited by 1,770
MECHANISMS AND BIOLOGICAL EFFECTS OF MISMATCH REPAIR
Modrich, P. (1991) · Annual Review of Genetics · doi:10.1146/annurev.ge.25.120191.001305 · Cited by 892
Self-citationDNA Mismatch Correction in a Defined System
Lahue, R. S., Au, K. G., & Modrich, P. (1989) · Science · doi:10.1126/science.2665076 · Cited by 559
Self-citationLoeb, L. A., & Kunkel, T. A. (1982) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.51.070182.002241 · Cited by 511
Holmes, J., Clark, S., & Modrich, P. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.15.5837 · Cited by 405
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Metadata
- Authors
- Lu, A. L., Clark, S., & Modrich, P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1983-08
- Volume
- 80
- Issue
- 15
- Pages
- 4639-4643
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2140852958
- Cited by
- 342
- References
- 21
- FWCI
- 9.20
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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