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Cited by 158

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Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids.

Open access
Thompson, C J, Gray, G S·1983·Proceedings of the National Academy of Sciences

Abstract

The DNA sequence of an aminoglycoside phosphotransferase gene (aph) from Streptomyces fradiae ATCC 10745 (a neomycin producer) was determined. The gene was localized by in vitro subcloning and insertional inactivation. Molecular weight, amino acid composition, and amino-terminal analysis of the purified aph gene product confirmed the accuracy and position of the aph gene sequence. Pairwise comparisons of S. fradiae aph with the aph genes encoded by bacterial transposons Tn5 and Tn903 showed significant nucleotide and amino acid homologies which indicated a common evolutionary origin for these antibiotic-resistance genes.

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Crossref · 10.1073/pnas.80.17.5190

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Metadata

Authors
Thompson, C. J., & Gray, G. S.
Venue
Proceedings of the National Academy of Sciences
Published
1983-09
Volume
80
Issue
17
Pages
5190-5194
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2072885047
Cited by
158
References
1
FWCI
11.14
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids. https://app.cochat.ai/papers/10.1073/pnas.80.17.5190
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Thompson, C. J., & Gray, G. S. (1983). Nucleotide sequence of a streptomycete aminoglycoside phosphotransferase gene and its relationship to phosphotransferases encoded by resistance plasmids. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.80.17.5190

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.