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Homonuclear 1H double-resonance difference spectroscopy of the rat brain in vivo.
Open accessAbstract
We have used 1H homonuclear double-resonance difference spectroscopy at 360.13 MHz to resolve specific metabolite resonances in the brains of intact rats. Metabolite resonances resolved include previously obscured proton resonances of alanine, gamma-aminobutyric acid (GABA), glutamate, and taurine. The gamma-aminobutyric acid alpha- and gamma-CH2 proton resonances were observed in the living rat in the difference spectrum obtained upon irradiation of the beta-CH2 proton resonance at 1.91 ppm. A 3-fold increase in the intensity of the alpha- and gamma-CH2 resonances of gamma-aminobutyric acid was observed 30 min after death. The alpha-CH and gamma-CH2 resonances of glutamate were also resolved in vivo by selective irradiation of the beta-CH2 protons to which they are spin-coupled. In addition, this technique was used to observe the beta-CH3 protons of lactate through the intact scalp of a rat. Large lipid signals arising from scalp tissue were eliminated in the difference spectrum, revealing the lactate beta-CH3 resonance.
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Cited by
Simultaneousin vivo spectral editing and water suppression
Mescher, M., Merkle, H., Kirsch, J., Garwood, M., & Gruetter, R. (1998) · NMR in Biomedicine · doi:10.1002/(sici)1099-1492(199810)11:6<266::aid-nbm530>3.0.co;2-j · Cited by 1,015
Rothman, D. L., Behar, K. L., Hyder, F., & Shulman, R. G. (2003) · Annual Review of Physiology · doi:10.1146/annurev.physiol.65.092101.142131 · Cited by 337
Self-citationThe effect of gabapentin on brain gamma‐aminobutyric acid in patients with epilepsy
Petroff, O. A. C., Rothman, D. L., Behar, K. L., Lamoureux, D., & Mattson, R. H. (1996) · Annals of Neurology · doi:10.1002/ana.410390114 · Cited by 285
Self-citationGlutamate and glutamine: a review of in vivo MRS in the human brain
Ramadan, S., Lin, A., & Stanwell, P. (2013) · NMR in Biomedicine · doi:10.1002/nbm.3045 · Cited by 282
Kapogiannis, D., Reiter, D. A., Willette, A. A., & Mattson, M. P. (2013) · NeuroImage · doi:10.1016/j.neuroimage.2012.09.029 · Cited by 211
Metadata
- Authors
- Rothman, D. L., Behar, K. L., Hetherington, H. P., & Shulman, R. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1984-10
- Volume
- 81
- Issue
- 20
- Pages
- 6330-6334
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2031229650
- Cited by
- 209
- References
- 0
- FWCI
- 9.61
- Type
- journal article
- Language
- en
- Open access
- green
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- Rothman, D. L., Behar, K. L., Hetherington, H. P., & Shulman, R. G. (1984). Homonuclear 1H double-resonance difference spectroscopy of the rat brain in vivo. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.20.6330
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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