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Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix.
Open accessAbstract
The culture conditions found to result in stable proliferation of normal rat hepatocytes are: (i) subconfluent cell densities; (ii) serum-free medium; (iii) hormonally defined medium containing epidermal growth factor, insulin, glucagon, prolactin, and other growth factors; and (iv) substrata of liver extracellular matrix depleted of growth inhibitors. Serum was found deleterious to parenchymal cells: it was inhibitory to the expression of liver-specific functions, cytostatic to parenchymal cells at all seeding densities, and cytotoxic to them at low seeding densities. These studies emphasize the relevance of synergies in the influences of hormones and extracellular matrix in regulating hepatocellular physiology.
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Cited by
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LeCluyse, E. L., Witek, R. P., Andersen, M. E., & Powers, M. J. (2012) · Critical Reviews in Toxicology · doi:10.3109/10408444.2012.682115 · Cited by 363
Kubota, H., Avarbock, M. R., & Brinster, R. L. (2004) · Biology of Reproduction · doi:10.1095/biolreprod.104.029207 · Cited by 344
GATA transcription factors as potentiators of gut endoderm differentiation
Bossard, P., & Zaret, K. S. (1998) · Development · doi:10.1242/dev.125.24.4909 · Cited by 313
Zhang, Y., He, Y., Bharadwaj, S., Hammam, N., Carnagey, K., Myers, R., Atala, A., & Van Dyke, M. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.04.005 · Cited by 276
Kidambi, S., Yarmush, R. S., Novik, E., Chao, P., Yarmush, M. L., & Nahmias, Y. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0906820106 · Cited by 213
Metadata
- Authors
- Enat, R., Jefferson, D. M., Ruiz-Opazo, N., Gatmaitan, Z., Leinwand, L. A., & Reid, L. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1984-03
- Volume
- 81
- Issue
- 5
- Pages
- 1411-1415
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2088227793
- Cited by
- 362
- References
- 0
- FWCI
- 21.34
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix. https://app.cochat.ai/papers/10.1073/pnas.81.5.1411
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- Enat, R., Jefferson, D. M., Ruiz-Opazo, N., Gatmaitan, Z., Leinwand, L. A., & Reid, L. M. (1984). Hepatocyte proliferation in vitro: its dependence on the use of serum-free hormonally defined medium and substrata of extracellular matrix. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.81.5.1411
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
