Glycolysis and methylaminoisobutyrate uptake in rat-1 cells transfected with ras or myc oncogenes.
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A high rate of aerobic glycolysis was catalyzed by rat-1 cells transfected with a ras oncogene (ras cells); rat-1 cells and rat-1 cells transfected with myc oncogene (myc cells) showed a low rate of glycolysis that was increased after exposure of the cells to type B transforming growth factor (TGF-beta). The uptake of radioactive methylaminoisobutyric acid or L-methionine via system A of amino acid transport also was accelerated after exposure of these cells to TGF-beta, with the myc cells being most sensitive and the ras cells least sensitive. Methionine was found to be a potent inhibitor of glycolysis in ras cells as well as in rat-1 or myc cells that were exposed to TGF-beta. We propose a relationship between the product of the ras oncogene (p21) and the protein(s) induced by exposure to TGF-beta.
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Oncogenic KRAS upregulates PFK1, ENO1, and LDHA, promoting glycolytic flux and production of intermediates that can enter anabolic pathways.
“Oncogenic KRAS also upregulates expression of other key glycolytic enzymes, such as PFK1, ENO1, and LDHA28,31–33, thereby promoting glycolytic flux and enabling the production of glycolytic intermediates that can be shunted into other anabolic pathways (Fig 2).”
From The metabolic landscape of RAS-driven cancers from biology to therapy (2021) · cites this paper as [32]
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- Authors
- Racker, E., Resnick, R. J., & Feldman, R.
- Affiliations
- Cornell University (US)
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1985-06
- Volume
- 82
- Issue
- 11
- Pages
- 3535-3538
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC397819
- OpenAlex
- W2049617964
- Cited by
- 135
- References
- 16
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 2 · 2023: 10 · 2022: 6 · 2021: 6 · 2020: 7 · 2019: 7 · 2018: 7 · 2017: 1 · 2016: 4 · 2015: 2 · 2014: 9 · 2013: 4 · 2012: 5
- FWCI
- 4.64
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (CA-08964)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Cancer, Hypoxia, and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › TGF-β signaling in diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Keywords
- aerobic glycolysis, system A amino acid transport, oncogenic Ras, MYC oncogene, p21ras, oncogene-transformed cells, Rat-1 cells
- MeSH terms
- Aminoisobutyric Acids, Animals, Biological Transport, Active, Cell Transformation, Neoplastic, Cycloheximide, Dose-Response Relationship, Drug, Fibroblasts, Glycolysis, Methionine, Oncogenes, Peptides, Platelet-Derived Growth Factor, Transfection, Transforming Growth Factors, Rats
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