The DOI matches its Crossref record; 23 of the 23 references it cites were found in a registry or an index.
Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.
Open accessAbstract
We describe a new assay system that allows a rapid, direct, and quantitative detection of promoter-dependent in vitro transcription by RNA polymerase II. The template used is a hybrid plasmid containing the adenovirus major late promoter linked to a synthetic 400-base-pair DNA fragment that lacks cytidine residues on the transcribed strand--i.e., generates a transcript with no guanosine residues. In vitro transcriptions are carried out in the absence of GTP or, if the reactions contain GTP, in the presence of RNase T1 and the chain terminator 3'-0-methyl-GTP. Under these conditions the only RNAs that can accumulate, whether from a circular or linearized DNA template, are the 400-nucleotide RNase T1-resistant transcripts resulting from accurate initiation at the major late promoter. Thus, specific transcription can be directly monitored by conventional RNA quantitation methods. Using this fast assay, we show that three basic transcription factors, TFIIB, TFIID, and TFIIE, are absolutely required, in addition to the RNA polymerase II, for specific transcription initiation from the adenovirus major late promoter. Units of activity can be defined for each of these individual components. The applicability of this kind of assay to other systems is discussed.
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Cites
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Self-citationDNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.
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[36] Eukaryotic gene transcription with purified components
Dignam, J. D., Martin, P. L., Shastry, B. S., & Roeder, R. G. (1983) · Methods in Enzymology · doi:10.1016/0076-6879(83)01039-3 · Cited by 963
Self-citationDynan, W. S., & Tjian, R. (1983) · Cell · doi:10.1016/0092-8674(83)90053-3 · Cited by 732
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Self-citationMultiple factors required for accurate initiation of transcription by purified RNA polymerase II.
Matsui, T., Segall, J., Weil, P. A., & Roeder, R. G. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)70232-4 · Cited by 530
Self-citationA drosophila RNA polymerase II transcription factor binds to the regulatory site of an hsp 70 gene
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Cited by
Purification and biochemical characterization of the promoter-specific transcription factor, Sp1
Briggs, M. R., Kadonaga, J. T., Bell, S. P., & Tjian, R. (1986) · Science · doi:10.1126/science.3529394 · Cited by 1,435
Tissue-specific in vitro transcription from the mouse albumin promoter
Gorski, K., Carneiro, M., & Schibler, U. (1986) · Cell · doi:10.1016/0092-8674(86)90519-2 · Cited by 1,253
Yamaguchi, Y., Takagi, T., Wada, T., Yano, K., Furuya, A., Sugimoto, S., Hasegawa, J., & Handa, H. (1999) · Cell · doi:10.1016/s0092-8674(00)80713-8 · Cited by 841
Stringer, K. F., Ingles, C. J., & Greenblatt, J. (1990) · Nature · doi:10.1038/345783a0 · Cited by 579
A High-Efficiency HeLa Cell Nuclear Transcription Extract
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Metadata
- Authors
- Sawadogo, M., & Roeder, R. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-07
- Volume
- 82
- Issue
- 13
- Pages
- 4394-4398
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1977694232
- Cited by
- 552
- References
- 23
- FWCI
- 9.19
- Type
- journal article
- Language
- en
- Open access
- green
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- Sawadogo, M., & Roeder, R. G. (1985). Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.13.4394
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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