The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
A High-Efficiency HeLa Cell Nuclear Transcription Extract
Abstract
A HeLa cell nuclear transcription extract that is approximately 20 times more efficient than standard HeLa cell transcription extracts was developed. Transcription of the strong adenovirus II major late promoter by this extract results in the synthesis of 1.5-4 molecules of product RNA per molecule of template, indicating that the extract is capable of multiple rounds of initiation. Standard HeLa cell nuclear extracts transcribe closed circular and linear adenovirus major late promoter templates with equal efficiency. In contrast, the new extract exhibits an increase of approximately twofold on transcription of a closed circular, as opposed to a linear, major late promoter template.
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Cites
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Self-citation[35] In Vitro transcription: Whole-cell extract
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Cited by
Yoshida, H., Okada, T., Haze, K., Yanagi, H., Yura, T., Negishi, M., & Mori, K. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.18.6755-6767.2000 · Cited by 934
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A common trans-acting factor, Ad4-binding protein, to the promoters of steroidogenic P-450s.
Morohashi, K., Honda, S., Inomata, Y., Handa, H., & Omura, T. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)37129-7 · Cited by 542
Ito, M., Yu, R., & Jameson, J. L. (1997) · Molecular and Cellular Biology · doi:10.1128/mcb.17.3.1476 · Cited by 462
Metadata
- Authors
- SHAPIRO, D. J., SHARP, P. A., WAHLI, W. W., & KELLER, M. J.
- Venue
- DNA
- Publisher
- SAGE Publications
- Published
- 1988-01
- Volume
- 7
- Issue
- 1
- Pages
- 47-55
- ISSN
- 0198-0238 (ISSN-L)
- OpenAlex
- W2083490046
- Cited by
- 577
- References
- 20
- FWCI
- 15.63
- Type
- journal article
- Language
- en
- Open access
- closed
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- SHAPIRO, D. J., SHARP, P. A., WAHLI, W. W., & KELLER, M. J. (1988). A High-Efficiency HeLa Cell Nuclear Transcription Extract. DNA. https://doi.org/10.1089/dna.1988.7.47
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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