The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A method to detect and characterize point mutations in transcribed genes: amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells.
Open accessAbstract
A significant percentage of human tumors contain activated ras oncogenes that have acquired oncogenic potential as a result of somatic point mutations at codon 12 or 61 of the encoded ras gene product. We report here a method to detect and characterize mutations in ras genes that is based on the ability of pancreatic ribonuclease (RNase A; EC 3.1.27.5) to cleave RNA heteroduplexes containing single-base mismatches. Using this method, we show that certain human tumor cells contain mutant c-Ki-ras genes, and we define the nature and position of these mutations. At the same time, we describe the presence and estimate the expression of both normal and mutant c-Ki-ras alleles in the same tumor cells. This method should be useful for the diagnostic detection and characterization of single point mutations in expressed genes.
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Cites
Nakano, H., Yamamoto, F., Neville, C., Evans, D., Mizuno, T., & Perucho, M. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.1.71 · Cited by 133
Self-citation
Cited by
Orita, M., Suzuki, Y., Sekiya, T., & Hayashi, K. (1989) · Genomics · doi:10.1016/0888-7543(89)90129-8 · Cited by 3,434
Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes
Almoguera, C., Shibata, D., Forrester, K., Martin, J., Arnheim, N., & Perucho, M. (1988) · Cell · doi:10.1016/0092-8674(88)90571-5 · Cited by 2,114
Self-citationStructural alterations of the epidermal growth factor receptor gene in human gliomas.
Wong, A. J., Ruppert, J. M., Bigner, S. H., Grzeschik, C. H., Humphrey, P. A., Bigner, D. S., & Vogelstein, B. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.7.2965 · Cited by 853
Tonon, G., Modi, S., Wu, L., Kubo, A., Coxon, A. B., Komiya, T., O'Neil, K., Stover, K., El-Naggar, A., Griffin, J. D., et al. (2003) · Nature Genetics · doi:10.1038/ng1083 · Cited by 567
The GLI-Kruppel family of human genes.
Ruppert, J. M., Kinzler, K. W., Wong, A. J., Bigner, S. H., Kao, F. T., Law, M. L., Seuanez, H. N., O'Brien, S. J., & Vogelstein, B. (1988) · Molecular and Cellular Biology · doi:10.1128/mcb.8.8.3104 · Cited by 299
Metadata
- Authors
- Winter, E., Yamamoto, F., Almoguera, C., & Perucho, M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-11
- Volume
- 82
- Issue
- 22
- Pages
- 7575-7579
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2053501297
- Cited by
- 316
- References
- 1
- FWCI
- 8.90
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: A method to detect and characterize point mutations in transcribed genes: amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells. https://app.cochat.ai/papers/10.1073/pnas.82.22.7575
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- Winter, E., Yamamoto, F., Almoguera, C., & Perucho, M. (1985). A method to detect and characterize point mutations in transcribed genes: amplification and overexpression of the mutant c-Ki-ras allele in human tumor cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.22.7575
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