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Cited by 1,126Immune Response and InflammationNitric Oxide and Endothelin EffectsNeutrophil, Myeloperoxidase and Oxidative Mechanisms

The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.

Mammalian nitrate biosynthesis: mouse macrophages produce nitrite and nitrate in response to Escherichia coli lipopolysaccharide.

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Stuehr, D J, Marletta, M A·1985·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.82.22.7738PMC PMC391409
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Abstract

Escherichia coli lipopolysaccharide (LPS)-induced nitrate biosynthesis was studied in LPS-sensitive C3H/He and LPS-resistant C3H/HeJ mice. Intraperitoneal injection of 15 micrograms of LPS led to a temporary 5- to 6-fold increase in blood nitrate concentration in the C3H/He strain. Levels of nitrate excreted in the urine were also increased. In contrast, no increase was observed in the C3H/HeJ strain with LPS injections up to 175 micrograms. Furthermore, thioglycolate-elicited peritoneal macrophages from C3H/He, but not from C3H/HeJ mice, produced nitrite (60%) and nitrate (40%) when cultured with LPS (10 micrograms/ml). T-lymphocyte addition/depletion experiments showed the presence of T cells enhanced this response. However, LPS did not cause nitrite or nitrate production in cultures of spleen lymphocytes from either strain. LPS-induced nitrate synthesis was also observed with nude mice and CBA/N mice, indicating that neither functional T lymphocytes nor LPS-responsive B lymphocytes were required for the response in vivo. This was consistent with the in vitro results showing macrophages alone were competent. Mycobacterium bovis infection of C3H/He and C3H/HeJ mice resulted in a large increase in nitrate production over the course of the infection for both strains, suggesting T-lymphocyte-mediated activation of macrophages as a potent stimulus for nitrate biosynthesis. The synthesis of nitrite is significant in that it can directly participate in the endogenous formation of nitrosamines and may also be involved in some aspect of the chemistry of cytotoxicity.

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  1. [2]Green, L. C., Ruiz De Luzuriaga, K., Wagner, D. A., Rand, W., Istfan, N., Young, V. R. & Tannenbaum, S. R. (1981) Proc. Natl. Acad. Sci. USA 78, 7764–7768.
  2. [7]Juhasz, L., Hill, M. J. & Nagy, G. (1978) in Environmental Aspects of N-Nitroso Compounds, eds. Walker, E. A., Castegnaro, M., Griciute, L. & Lyle, R. E. (Int. Agency Res. Cancer, Lyon, France), Publ. 19, pp. 619–623.
  3. [12]Wagner, D. A., Young, V. R. & Tannenbaum, S. R. (1983) Proc. Natl. Acad. Sci. USA 80, 4518–4521.
  4. [16]Senterfitt, V. C. & Shands, J. W. (1978) Proc. Soc. Exp. Biol. Med. 159, 69–74.
  5. [17]Mishell, B. B. & Shiigi, S. M. (1980) Selected Methods in Cellular Immunology (Freeman, San Francisco), pp. 4–6.
  6. [22]Scher, I. (1981) in Immunologic Defects in Laboratory Animals, ed. Gershwin, M. E. (Plenum, New York), Vol. 1, pp. 163-190.
  7. [21]Scher, I., Zaldivar, N. M. & Mosier, D. E. (1977) in Microbiology 1977, ed. Schlesinger, D. (Am. Soc. Microbiol., Washington, DC), pp. 310–313.
  8. [25]Vogel, S. N. & Rosenstreich, D. L. (1981) in Lymphokines, ed. Pick, E. (Academic, New York), Vol. 3, pp. 149–180.
  9. [26]Scibienski, R. J. (1981) in Immunologic Defects in Laboratory Animals, ed. Gershwin, M. E. (Plenum, New York), Vol. 2, pp. 241–258.
  10. [32]Kindred, B. (1981) in Immunologic Defects in Laboratory Animals, ed. Gershwin, M. E. (Plenum, New York), Vol. 1, pp. 215–265.
  11. [44]Gemsa, D. (1981) in Lymphokines, ed. Pick, E. (Academic, New York), Vol. 3, pp. 33–56.

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Metadata

Authors
Stuehr, D. J., & Marletta, M. A.
Affiliations
Massachusetts Institute of Technology (US)
ORCID
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1985-11
Volume
82
Issue
22
Pages
7738-7742
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.82.22.7738
PMC
PMC391409
OpenAlex
W1984997174
Cited by
1,126
References
35
Citations by year
2026: 6 · 2025: 4 · 2024: 10 · 2023: 8 · 2022: 13 · 2021: 11 · 2020: 17 · 2019: 14 · 2018: 18 · 2017: 18 · 2016: 7 · 2015: 16 · 2014: 12 · 2013: 13 · 2012: 31
FWCI
3.77
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Cancer Institute (CA-26731)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
  • Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Keywords
nitrite, macrophages, lipopolysaccharide, Escherichia coli, C3H/He mice, C3H/HeJ mice, T cells, peritoneal macrophages, nitrosamines, cytotoxicity
MeSH terms
Animals, BCG Vaccine, Escherichia coli, Lipopolysaccharides, Macrophage Activation, Macrophages, Mice, Inbred BALB C, Mice, Inbred CBA, Mice, Inbred C3H, Nitrates, Nitrites, T-Lymphocytes, Mice

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