CoChat
Sign Up
Cited by 65

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Evolution of apolipoprotein E: mouse sequence and evidence for an 11-nucleotide ancestral unit

Rajavashisth, T B, Kaptein, J S, Reue, K L, Lusis, A J·1985·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.82.23.8085
Open this paper in CoChat — free account

Abstract

Apolipoprotein E (apo E) is responsible for the binding of very low density lipoprotein and chylomicron remnants to cellular receptors thereby removing them from circulation. We have isolated and determined the sequence of a cDNA encoding 285 amino acids and the entire 3' untranslated region of 112 nucleotides of mouse apo E. The remaining coding sequence was determined by sequencing mouse liver mRNA. Comparisons with rat and human apo E sequences showed a high degree of conservation although there were regions in each species that were characterized by unique insertions and deletions. Analysis of the sequence homologies within apo E revealed that the entire sequence is made up of repetitive units. The most primitive unit appeared to be an 11-nucleotide repeat within higher order repeats of 22 or 33 nucleotides. The 11-nucleotide unit -TCGGACGAGGC- is read in all three reading frames, and when tandemly repeated, it encodes the highly conserved amino acid sequence Xaa-(Glu/Asp)-(Glu/Asp)-Xaa-Arg-Xaa-Arg-Leu-Gly-Xaa-Xaa. We postulate that apo E and those other apolipoproteins related to it have arisen by duplications and subsequent modifications of this or a closely related 11-nucleotide ancestral sequence.

Overview

Integrity signals

Registry

Crossref · 10.1073/pnas.82.23.8085

Cites

3 of 3 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Humans express three primary apoE isoforms that vary at only two of the 299 amino acids; mice express one form of apoE, the same as apoE4 at residues Arg112/Arg158, although homology between mouse and human apoE is only 70%.

    “While humans express three primary isoforms that vary at only two of the 299 amino acids, mice express one form of apoE, the same as apoE4 at residues Arg112/Arg158, although the homology between m- and h-apoE is only 70% [118].”

    From Inhibition of ACAT as a Therapeutic Target for Alzheimer's Disease Is Independent of ApoE4 Lipidation (2023) · cites this paper as [118]

Citations

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Metadata

Authors
Rajavashisth, T. B., Kaptein, J. S., Reue, K. L., & Lusis, A. J.
Venue
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences
Published
1985-12
Volume
82
Issue
23
Pages
8085-8089
ISSN
  • 0027-8424
  • 1091-6490
DOI
10.1073/pnas.82.23.8085
Cited by
65
References
3
Language
en

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review