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Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.
Open accessAbstract
An oligonucleotide hybridization procedure has been developed that eliminates the preferential melting of A X T versus G X C base pairs, allowing the stringency of the hybridization to be controlled as a function of probe length only. This technique, which uses tetramethylammonium chloride, is especially helpful whenever a highly complex library is screened with a pool of oligonucleotide probes, which usually vary widely in base composition. The procedure can also be applied advantageously whenever an exact match to an oligonucleotide probe is desired, such as in screening for clones having as little as a single-base alteration generated by in vitro mutagenesis.
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Cited by
Hirano, T., Yasukawa, K., Harada, H., Taga, T., Watanabe, Y., Matsuda, T., Kashiwamura, S.-I., Nakajima, K., Koyama, K., Iwamatsu, A., et al. (1986) · Nature · doi:10.1038/324073a0 · Cited by 2,139
Positional cloning of zebrafish ferroportin1 identifies a conserved vertebrate iron exporter
Donovan, A., Brownlie, A., Zhou, Y., Shepard, J., Pratt, S. J., Moynihan, J., Paw, B. H., Drejer, A., Barut, B., Zapata, A., et al. (2000) · Nature · doi:10.1038/35001596 · Cited by 1,667
Goedert, M., Wischik, C. M., Crowther, R. A., Walker, J. E., & Klug, A. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.11.4051 · Cited by 1,134
Kieran, M., Blank, V., Logeat, F., Vandekerckhove, J., Lottspeich, F., Le Bail, O., Urban, M. B., Kourilsky, P., Baeuerle, P. A., & Israël, A. (1990) · Cell · doi:10.1016/0092-8674(90)90275-j · Cited by 874
Advanced multiplexed analysis with the FlowMetrixTM system
Fulton, R. J., McDade, R. L., Smith, P. L., Kienker, L. J., & Kettman, J. R. (1997) · Clinical Chemistry · doi:10.1093/clinchem/43.9.1749 · Cited by 650
Metadata
- Authors
- Wood, W. I., Gitschier, J., Lasky, L. A., & Lawn, R. M.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1985-03
- Volume
- 82
- Issue
- 6
- Pages
- 1585-1588
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2032413102
- Cited by
- 790
- References
- 0
- FWCI
- 21.15
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. https://app.cochat.ai/papers/10.1073/pnas.82.6.1585
- The paper
- Wood, W. I., Gitschier, J., Lasky, L. A., & Lawn, R. M. (1985). Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.82.6.1585
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
