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Cited by 461

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Efficient cloning of genes of Neurospora crassa

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Vollmer, Steven J., Yanofsky, Charles·1986·Proceedings of the National Academy of Sciences
DOI 10.1073/pnas.83.13.4869
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Abstract

We have constructed a genomic library of Neurospora crassa DNA in a cosmid vector that contains the dominant selectable marker for benomyl resistance. The library is arranged to permit the rapid cloning of Neurospora genes by either sib-selection or colony-hybridization protocols. Detailed procedures for the uses of the library are described. By use of these procedures, a modest number of unrelated genes have been isolated. The cloning of trp-3 , the structural gene for the multifunctional enzyme tryptophan synthetase (tryptophan synthase, EC 4.2.1.20), is reported in detail; its identity was verified by restriction fragment length polymorphism mapping. The strategies described in this paper should be of use in the cloning of any gene of Neurospora , as well as genes of other lower eukaryotes.

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Metadata

Authors
Vollmer, S. J., & Yanofsky, C.
Venue
Proceedings of the National Academy of Sciences
Publisher
Proceedings of the National Academy of Sciences
Published
1986-07
Volume
83
Issue
13
Pages
4869-4873
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
DOI
10.1073/pnas.83.13.4869
OpenAlex
W1986657721
Cited by
461
References
0
FWCI
10.08
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Efficient cloning of genes of Neurospora crassa. https://app.cochat.ai/papers/10.1073/pnas.83.13.4869
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Vollmer, S. J., & Yanofsky, C. (1986). Efficient cloning of genes of Neurospora crassa. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.13.4869

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.