The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.
Open accessAbstract
DNA coding for bacteriophage T7 RNA polymerase was ligated to a vaccinia virus transcriptional promoter and integrated within the vaccinia virus genome. The recombinant vaccinia virus retained infectivity and stably expressed T7 RNA polymerase in mammalian cells. Target genes were constructed by inserting DNA segments that code for beta-galactosidase or chloramphenicol acetyltransferase into a plasmid with bacteriophage T7 promoter and terminator regions. When cells were infected with the recombinant vaccinia virus and transfected with plasmids containing the target genes, the latter were expressed at high levels. Chloramphenicol acetyltransferase activity was 400-600 times greater than that observed with conventional mammalian transient-expression systems regulated either by the enhancer and promoter regions of the Rous sarcoma virus long terminal repeat or by the simian virus 40 early region. The vaccinia/T7 hybrid virus forms the basis of a simple, rapid, widely applicable, and efficient mammalian expression system.
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A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
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Localization of low molecular weight GTP binding proteins to exocytic and endocytic compartments
Chavrier, P., Parton, R. G., Hauri, H. P., Simons, K., & Zerial, M. (1990) · Cell · doi:10.1016/0092-8674(90)90369-p · Cited by 1,166
Inhibition of rab5 GTPase activity stimulates membrane fusion in endocytosis.
Stenmark, H., Parton, R. G., Steele‐Mortimer, O., Lütcke, A., Gruenberg, J., & Zerial, M. (1994) · The EMBO Journal · doi:10.1002/j.1460-2075.1994.tb06381.x · Cited by 968
Metadata
- Authors
- Fuerst, T. R., Niles, E. G., Studier, F. W., & Moss, B.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-11
- Volume
- 83
- Issue
- 21
- Pages
- 8122-8126
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2005411001
- Cited by
- 2,089
- References
- 1
- FWCI
- 5.11
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Fuerst, T. R., Niles, E. G., Studier, F. W., & Moss, B. (1986). Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.21.8122
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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