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Cloning and expression of human lecithin-cholesterol acyltransferase cDNA.
Open accessAbstract
cDNA and genomic cloning has been used to determine the mRNA and amino acid sequence of human plasma lecithin-cholesterol acyltransferase (LCATase; EC 2.3.1.43). The mature protein was found to contain 416 amino acid residues with a hydrophobic leader sequence of 24 amino acids. An unusual feature of the message is that the poly(A) signal AATAAA overlaps the COOH-terminal glutamic acid and stop codons, and the 3' untranslated region is only 23 bases. The protein itself is distinguished by a number of extended sequences of hydrophobic amino acids, one of which contains a hexapeptide identical with the interfacial binding segment of the active site of pancreatic lipase and is similar to the same site of lingual lipase. The cloned cDNA allows the expression of active LCATase by transfected tissue culture cells.
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Citations
Cited by
Molecular physiology of reverse cholesterol transport.
Fielding, C. J., & Fielding, P. E. (1995) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)39898-9 · Cited by 1,448
Self-citationThe Metabolic Serine Hydrolases and Their Functions in Mammalian Physiology and Disease
Long, J. Z., & Cravatt, B. F. (2011) · Chemical Reviews · doi:10.1021/cr200075y · Cited by 425
The molecular pathology of lecithin:cholesterol acyltransferase (LCAT) deficiency syndromes
Kuivenhoven, J. A., Pritchard, H., Hill, J., Frohlich, J., Assmann, G., & Kastelein, J. (1997) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)37433-2 · Cited by 367
The sequence of cDNA encoding lipoprotein lipase. A member of a lipase gene family.
Kirchgessner, T. G., Svenson, K. L., Lusis, A. J., & Schotz, M. C. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)47435-2 · Cited by 297
Haase, C. L., Tybjærg-Hansen, A., Ali Qayyum, A., Schou, J., Nordestgaard, B. G., & Frikke-Schmidt, R. (2012) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-1846 · Cited by 282
Metadata
- Authors
- McLean, J., Fielding, C., Drayna, D., Dieplinger, H., Baer, B., Kohr, W., Henzel, W., & Lawn, R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-04
- Volume
- 83
- Issue
- 8
- Pages
- 2335-2339
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2033168374
- Cited by
- 164
- References
- 0
- FWCI
- 19.44
- Type
- journal article
- Language
- en
- Open access
- green
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- McLean, J., Fielding, C., Drayna, D., Dieplinger, H., Baer, B., Kohr, W., Henzel, W., & Lawn, R. (1986). Cloning and expression of human lecithin-cholesterol acyltransferase cDNA. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.8.2335
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