The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
Structure of the high-affinity binding site for noncompetitive blockers of the acetylcholine receptor: serine-262 of the delta subunit is labeled by [3H]chlorpromazine.
Open accessAbstract
The membrane-bound acetylcholine receptor from Torpedo marmorata was photolabeled by the noncompetitive channel blocker [3H]chlorpromazine under equilibrium conditions in the presence of agonist. Incorporation of radioactivity into all subunits occurred and was reduced by addition of phencyclidine, a specific ligand for the high-affinity site for noncompetitive blockers. The delta subunit was purified and digested with trypsin, and the resulting fragments were fractionated by reversed-phase HPLC. The labeled peptide could not be purified to homogeneity because of its marked hydrophobic character, but a combination of differential CNBr subcleavage and cosequencing of partially purified fragments enabled us to identify Ser-262 as being labeled by [3H]chlorpromazine. The labeling of this particular residue was prevented by phencyclidine and thus took place at the level of, or in proximity to, the high-affinity site for noncompetitive blockers. Ser-262 is located in a hydrophobic and potentially transmembrane segment termed MII.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,221
Swank, R. T., & Munkres, K. D. (1971) · Analytical Biochemistry · doi:10.1016/0003-2697(71)90436-2 · Cited by 1,717
Structural homology of Torpedo californica acetylcholine receptor subunits
Noda, M., Takahashi, H., Tanabe, T., Toyosato, M., Kikyotani, S., Furutani, Y., Hirose, T., Takashima, H., Inayama, S., Miyata, T., et al. (1983) · Nature · doi:10.1038/302528a0 · Cited by 799
[36] High-sensitivity sequencing with a gas-phase sequenator
Hunkapiller, M. W., Hewick, R. M., Dreyer, W. J., & Hood, L. E. (1983) · Methods in Enzymology · doi:10.1016/s0076-6879(83)91038-8 · Cited by 659
Acetylcholine Receptor: An Allosteric Protein
Changeux, J.-P., Devillers-Thiéry, A., & Chemouilli, P. (1984) · Science · doi:10.1126/science.6382611 · Cited by 623
Self-citationNoda, M., Takahashi, H., Tanabe, T., Toyosato, M., Kikyotani, S., Hirose, T., Asai, M., Takashima, H., Inayama, S., Miyata, T., et al. (1983) · Nature · doi:10.1038/301251a0 · Cited by 530
Amphipathic analysis and possible formation of the ion channel in an acetylcholine receptor.
Finer-Moore, J., & Stroud, R. M. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.1.155 · Cited by 506
Kao, P. N., Dwork, A. J., Kaldany, R. R., Silver, M. L., Wideman, J., Stein, S., & Karlin, A. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(20)71257-3 · Cited by 410
Claudio, T., Ballivet, M., Patrick, J., & Heinemann, S. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.4.1111 · Cited by 409
Cited by
Macdonald, R. L., & Olsen, R. W. (1994) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.17.030194.003033 · Cited by 1,975
Membrane protein folding and oligomerization: the two-stage model
Popot, J. L., & Engelman, D. M. (1990) · Biochemistry · doi:10.1021/bi00469a001 · Cited by 932
Acetylcholine Receptor Channel Structure Probed in Cysteine-Substitution Mutants
Akabas, M. H., Stauffer, D. A., Xu, M., & Karlin, A. (1992) · Science · doi:10.1126/science.1384130 · Cited by 665
Toward a structural basis for the function of nicotinic acetylcholine receptors and their cousins
Karlin, A., & Akabas, M. H. (1995) · Neuron · doi:10.1016/0896-6273(95)90004-7 · Cited by 615
Nakanishi, N., Shneider, N. A., & Axel, R. (1990) · Neuron · doi:10.1016/0896-6273(90)90212-x · Cited by 595
Metadata
- Authors
- Giraudat, J., Dennis, M., Heidmann, T., Chang, J. Y., & Changeux, J. P.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1986-04
- Volume
- 83
- Issue
- 8
- Pages
- 2719-2723
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2006798674
- Cited by
- 301
- References
- 28
- FWCI
- 8.84
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Structure of the high-affinity binding site for noncompetitive blockers of the acetylcholine receptor: serine-262 of the delta subunit is labeled by [3H]chlorpromazine. https://app.cochat.ai/papers/10.1073/pnas.83.8.2719
- The paper
- Giraudat, J., Dennis, M., Heidmann, T., Chang, J. Y., & Changeux, J. P. (1986). Structure of the high-affinity binding site for noncompetitive blockers of the acetylcholine receptor: serine-262 of the delta subunit is labeled by [3H]chlorpromazine. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.83.8.2719
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
