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A human beta-actin expression vector system directs high-level accumulation of antisense transcripts.
Open accessAbstract
We have constructed a mammalian expression vector consisting of 3 kilobases of the human beta-actin gene 5' flanking sequence plus 5' untranslated region and intervening sequence 1 linked at the 3' splice site to a short DNA polylinker sequence containing unique Sal I, HindIII, and BamHI restriction endonuclease sites followed by a simian virus 40 (SV40) polyadenylylation signal. Two derivatives, containing the selection markers obtained from pSV2gpt or pSV2neo, were also generated. We find that the promoter activity of this vector is a great or greater than that of the SV40 early promoter in a variety of human and rodent cells. The vector was used to generate gamma-actin and beta-tubulin antisense transcripts in human fibroblast cell lines. The antisense transcripts accumulate to levels comparable with that of the highly abundant gamma-actin and beta-tubulin mRNAs.
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Metadata
- Authors
- Gunning, P., Leavitt, J., Muscat, G., Ng, S. Y., & Kedes, L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1987-07
- Volume
- 84
- Issue
- 14
- Pages
- 4831-4835
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2089559330
- Cited by
- 739
- References
- 0
- FWCI
- 8.19
- Type
- journal article
- Language
- en
- Open access
- green
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- Gunning, P., Leavitt, J., Muscat, G., Ng, S. Y., & Kedes, L. (1987). A human beta-actin expression vector system directs high-level accumulation of antisense transcripts. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.84.14.4831
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
