Cited by 367Acute Myeloid Leukemia ResearchRetinoids in leukemia and cellular processesGenomics and Chromatin Dynamics

The zinc finger transcription factor Egr-1 is essential for and restricts differentiation along the macrophage lineage

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Nguyen, Hung Q., Hoffman-Liebermann, Barbara, Liebermann, Dan A.·1993·Cell

Abstract

We have isolated cDNA clones of myeloid differentiation primary response (MyD) genes, activated in the absence of de novo protein synthesis following induction for differentiation along either the macrophage or granulocyte lineage in human myeloblastic leukemia HL-60 cells. One cDNA clone of a primary response gene, expressed upon macrophage differentiation, encoded for Egr-1, a zinc finger transcription factor. The Egr-1 gene was observed to be transcriptionally silent in HL-60 cells, but active in U-937 and M1 cells, the latter two being predetermined for macrophage differentiation. Egr-1 antisense oligomers in the culture media blocked macrophage differentiation in both myeloid leukemia cell lines and normal myeloblasts. HL-60 cells constitutively expressing an Egr-1 transgene (HL-60Egr-1) could be induced for macrophage, but not granulocyte, differentiation. These observations indicate that expression of Egr-1 is essential for and restricts differentiation of myeloblasts along the macrophage lineage.

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Crossref · 10.1016/0092-8674(93)90660-i

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Authors
Nguyen, H. Q., Hoffman-Liebermann, B., & Liebermann, D. A.
Affiliations
University of Pennsylvania (US)
Venue
Cell
Publisher
Elsevier BV
Published
1993-01
Volume
72
Issue
2
Pages
197-209
ISSN
0092-8674 (ISSN-L)
OpenAlex
W1993004730
Cited by
367
References
76
Citations by year
2026: 3 · 2025: 4 · 2024: 1 · 2023: 2 · 2022: 4 · 2021: 3 · 2020: 5 · 2019: 7 · 2018: 4 · 2017: 7 · 2016: 1 · 2015: 5 · 2014: 6 · 2013: 7 · 2012: 7
FWCI
21.10
Type
journal article
Language
en
Open access
Locations
  • Cell (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5r01ca043618-12)
  • National Cancer Institute (1RO1CA43618-01)
Topics
  • Health Sciences › Medicine › Hematology › Acute Myeloid Leukemia Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Retinoids in leukemia and cellular processes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Keywords
EGR1, zinc finger transcription factor, macrophage differentiation, granulocytic differentiation, HL-60 cells, U937 cells, M1 cells, antisense oligomers, transgene expression, myeloblasts, transcriptional regulation
MeSH terms
Animals, Base Sequence, Binding Sites, Bone Marrow, Bone Marrow Cells, Cell Differentiation, Cell Nucleus, Dimethyl Sulfoxide, DNA-Binding Proteins, DNA, Neoplasm, Granulocytes, Hematopoietic Stem Cells, Humans, Macrophages, Mice, Inbred Strains, Molecular Sequence Data, Poly A, RNA, RNA, Messenger, Tetradecanoylphorbol Acetate, Transcription Factors, Transcription, Genetic, Transfection, Tumor Cells, Cultured, Leukemia, Promyelocytic, Acute, Gene Expression, Zinc Fingers, Oligonucleotides, Antisense, Immediate-Early Proteins, Mice, Early Growth Response Protein 1

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