The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Molecular cloning, nucleotide sequence, and expression of the gene encoding human eosinophil differentiation factor (interleukin 5).
Open accessAbstract
The human eosinophil differentiation factor (EDF) gene was cloned from a genomic library in lambda phage EMBL3A by using a murine EDF cDNA clone as a probe. The DNA sequence of a 3.2-kilobase BamHI fragment spanning the gene was determined. The gene contains three introns. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin 5 but shows no significant homology with other known hemopoietic growth regulators. The amino acid sequence shows strong homology (approximately 70% identity) with that of murine EDF. Recombinant human EDF, expressed from the human EDF gene after transfection into monkey COS cells, stimulated the production of eosinophils and eosinophil colonies from normal human bone marrow but had no effect on the production of neutrophils or mononuclear cells (monocytes and lymphoid cells). The apparent specificity of human EDF for the eosinophil lineage in myeloid hemopoiesis contrasts with the properties of human interleukin 3 and granulocyte/macrophage and granulocyte colony-stimulating factors but is directly analogous to the biological properties of murine EDF. Human EDF therefore represents a distinct hemopoietic growth factor that could play a central role in the regulation of eosinophilia.
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Cites
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Cited by
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IMMUNOLOGIC BASIS OF ANTIGEN-INDUCED AIRWAY HYPERRESPONSIVENESS
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Metadata
- Authors
- Campbell, H. D., Tucker, W. Q., Hort, Y., Martinson, M. E., Mayo, G., Clutterbuck, E. J., Sanderson, C. J., & Young, I. G.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1987-10
- Volume
- 84
- Issue
- 19
- Pages
- 6629-6633
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2009081607
- Cited by
- 318
- References
- 26
- FWCI
- 21.31
- Type
- journal article
- Language
- en
- Open access
- green
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- Campbell, H. D., Tucker, W. Q., Hort, Y., Martinson, M. E., Mayo, G., Clutterbuck, E. J., Sanderson, C. J., & Young, I. G. (1987). Molecular cloning, nucleotide sequence, and expression of the gene encoding human eosinophil differentiation factor (interleukin 5). Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.84.19.6629
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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