The DOI matches its Crossref record; 33 of the 33 references it cites were found in a registry or an index.
Role of an adenovirus E2 promoter binding factor in E1A-mediated coordinate gene control.
Open accessAbstract
A product of the adenovirus gene E1A is responsible for the stimulation of transcription from six viral promoters as well as at least two cellular promoters. We have detected a HeLa cell factor, termed E2 promoter binding factor (E2F), that appears to mediate the transcriptional stimulation of the viral E2 promoter. Competition experiments revealed that E2F did not recognize and bind to the E1B, E3, E4, or major late promoter sequences. Furthermore, three additional promoters stimulated by E1A, heat shock protein 70, beta-globin, and early simian virus 40, do not bind E2F. In contrast, the factor does recognize sequences in the E1A enhancer, and within the E1A enhancer are duplicated binding sites for E2F. Finally, a single E2F binding site from the E1A enhancer can confer increased transcription to a mouse beta-globin promoter, dependent on the action of the E1A gene product. This stimulation requires binding of E2F since methylation of the binding site, which blocks binding in vitro, reduces transcription stimulation in vivo. We, therefore, conclude that E2F is likely to be responsible for the E1A-mediated stimulation of the E1A gene as well as the E2 gene but is not involved in the activation of the other E1A-inducible promoters.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The manipulation of the methylation status of DNA sequences has been shown (mostly in in vitro studies) to result in the differential binding of TFs, including E2F, AP2α, MYC and MYN88–95.”
From Transcription factors as readers and effectors of DNA methylation (2016) · cites this paper as [94]
Citations
Cites
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
Chirgwin, J. M., Przybyla, A., MacDonald, R. J., & Rutter, W. J. (1979) · Biochemistry · doi:10.1021/bi00591a005 · Cited by 21,460
Dignam, J. D., Lebovitz, R. M., & Roeder, R. G. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.5.1475 · Cited by 11,262
Southern, P. J., & Berg, P. R. (1982) · PubMed · Cited by 2,740
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis
Fried, M. G., & Crothers, D. M. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.23.6505 · Cited by 2,658
DNAase footprinting a simple method for the detection of protein-DNA binding specificity
Galas, D. J., & Schmitz, A. (1978) · Nucleic Acids Research · doi:10.1093/nar/5.9.3157 · Cited by 2,109
Garner, M. M., & Revzin, A. (1981) · Nucleic Acids Research · doi:10.1093/nar/9.13.3047 · Cited by 2,021
DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
Wigler, M. H., Pellicer, À., Silverstein, S. J., Axel, R., Urlaub, G., & Chasin, L. A. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.3.1373 · Cited by 1,278
DNA METHYLATION AND GENE ACTIVITY
Doerfler, W. (1983) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.52.070183.000521 · Cited by 1,094
An adenovirus type 5 early gene function regulates expression of other early viral genes.
Jones, N., & Shenk, T. E. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.8.3665 · Cited by 733
Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs
Berk, A. J., Lee, F., Harrison, T. J., Williams, J., & Sharp, P. A. (1979) · Cell · doi:10.1016/0092-8674(79)90333-7 · Cited by 693
Cited by
Sorted by citations
Frommer, M., McDonald, L. E., Millar, D. S., Collis, C. M., Watt, F., Grigg, G. W., Molloy, P. L., & Paul, C. L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.5.1827 · Cited by 3,238
Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding Proteins
Mitchell, P. J., & Tjian, R. (1989) · Science · doi:10.1126/science.2667136 · Cited by 3,046
The regulation of E2F by pRB-family proteins.
Dyson, N. (1998) · Genes & Development · doi:10.1101/gad.12.15.2245 · Cited by 2,317
E2F: a Link Between the Rb Tumor Suppressor Protein and Viral Oncoproteins
Nevins, J. R. (1992) · Science · doi:10.1126/science.1411535 · Cited by 1,506
Self-citationCompilation of vertebrate-encoded transcription factors
Faisst, S., & Meyer, S. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.1.3 · Cited by 902
Metadata
- Authors
- Kovesdi, I., Reichel, R., & Nevins, J. R.
- ORCID
- Kovesdi, I
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1987-04
- Volume
- 84
- Issue
- 8
- Pages
- 2180-2184
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC304612
- OpenAlex
- W1968812729
- Cited by
- 278
- References
- 31
- Citations by year
- 2026: 1 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 5 · 2017: 1 · 2016: 6 · 2015: 9 · 2014: 6 · 2013: 1 · 2012: 5
- FWCI
- 9.92
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM 26765)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
- Keywords
- adenovirus E1A, transcriptional activation, viral promoters, DNA binding sites, promoter methylation, beta-globin promoter, HeLa cells
- MeSH terms
- Adenoviruses, Human, Base Sequence, Cell Nucleus, Genes, Genes, Viral, HeLa Cells, Humans, Oncogene Proteins, Viral, Plasmids, Promoter Regions, Genetic, Transcription Factors, Transcription, Genetic, Adenovirus Early Proteins
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
