The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.
Open accessAbstract
Single-copy sequences can be enzymatically amplified from genomic DNA by the polymerase chain reaction. By using unequal molar amounts of the two amplification primers, it is possible in a single step to amplify a single-copy gene and produce an excess of single-stranded DNA of a chosen strand for direct sequencing or for use as a hybridization probe. Further, individual alleles in a heterozygote can be sequenced directly by using allele-specific oligonucleotides either in the amplification reaction or as sequencing primers. By using these methods, we have studied the allelic diversity at the HLA-DQA locus and its association with the serologically defined HLA-DR and -DQ types. This analysis has revealed a total of eight alleles and three additional haplotypes. This procedure has wide applications in screening for mutations in human genes and facilitates the linking of enzymatic amplification of genes to automated sequencing.
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Citations
Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Self-citationSaiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Self-citation
Cited by
Kocher, T. D., Thomas, W. K., Meyer, A., Edwards, S. V., Pääbo, S., Villablanca, F. X., & Wilson, A. C. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.16.6196 · Cited by 4,969
Orita, M., Suzuki, Y., Sekiya, T., & Hayashi, K. (1989) · Genomics · doi:10.1016/0888-7543(89)90129-8 · Cited by 3,434
Evolution of the cytochromeb gene of mammals
Irwin, D. M., Kocher, T. D., & Wilson, A. C. (1991) · Journal of Molecular Evolution · doi:10.1007/bf02515385 · Cited by 2,315
Goto, Y.-I., Nonaka, I., & Horai, S. (1990) · Nature · doi:10.1038/348651a0 · Cited by 2,070
Verge, C. F., Gianani, R., Kawasaki, E., Yu, L., Pietropaolo, M., Chase, H. P., Eisenbarth, G. S., & Jackson, R. A. (1996) · Diabetes · doi:10.2337/diab.45.7.926 · Cited by 827
Metadata
- Authors
- Gyllensten, U. B., & Erlich, H. A.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1988-10
- Volume
- 85
- Issue
- 20
- Pages
- 7652-7656
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2080824017
- Cited by
- 1,560
- References
- 2
- FWCI
- 54.00
- Type
- journal article
- Language
- en
- Open access
- green
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- Gyllensten, U. B., & Erlich, H. A. (1988). Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.85.20.7652
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