Cited by 259Cholesterol and Lipid MetabolismAtherosclerosis and Cardiovascular DiseasesLipoproteins and Cardiovascular Health

Purification and characterization of a bovine acetyl low density lipoprotein receptor.

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Kodama, T, Reddy, P, Kishimoto, C, Krieger, M·1988·Proceedings of the National Academy of Sciences

Abstract

The acetyl low density lipoprotein (LDL) receptor is expressed on macrophages and some endothelial cells and mediates macrophage-foam cell formation in culture. A 220-kDa acetyl LDL binding protein was partially purified from bovine liver membranes and was used to make a specific monoclonal antibody. The 220-kDa protein immunoprecipitated by this antibody retained binding activity, and the antibody was used to detect this protein in cells lining bovine liver sinusoids and on the surface of cultured bovine alveolar macrophages. In the human monocytic cell line THP-1, the expression of both acetyl LDL receptor activity and a 220-kDa acetyl LDL binding protein were dramatically induced in parallel after differentiation to a macrophage-like state induced by phorbol ester. The ligand specificity, tissue and cell-type specificity, and coinduction data indicated that this 220-kDa cell-surface binding protein is probably a receptor that mediates acetyl LDL endocytosis. The 220-kDa protein, which was purified 238,000-fold from bovine lung membranes to near homogeneity using monoclonal antibody affinity chromatography, is a trimer of 77-kDa subunits that contain asparagine-linked carbohydrate chains.

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Crossref · 10.1073/pnas.85.23.9238

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Authors
Kodama, T., Reddy, P., Kishimoto, C., & Krieger, M.
Affiliations
Massachusetts Institute of Technology (US)
Venue
Proceedings of the National Academy of Sciences
Published
1988-12
Volume
85
Issue
23
Pages
9238-9242
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2063813098
Cited by
259
References
33
Citations by year
2025: 2 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 4 · 2016: 5 · 2015: 3 · 2014: 6 · 2013: 3 · 2012: 3
FWCI
14.24
Type
journal article
Language
en
Open access
Locations
  • Proceedings of the National Academy of Sciences (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
  • Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
  • Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
Keywords
macrophage foam cell formation, monoclonal antibodies, receptor purification, phorbol ester, receptor internalization, asparagine-linked glycosylation, atherosclerosis
MeSH terms
Acetylation, Animals, Cattle, Cell Membrane, Chromatography, Affinity, Kinetics, Lipoproteins, LDL, Liver, Macrophages, Membrane Proteins, Receptors, LDL

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