Purification and characterization of a bovine acetyl low density lipoprotein receptor.
Open accessAbstract
The acetyl low density lipoprotein (LDL) receptor is expressed on macrophages and some endothelial cells and mediates macrophage-foam cell formation in culture. A 220-kDa acetyl LDL binding protein was partially purified from bovine liver membranes and was used to make a specific monoclonal antibody. The 220-kDa protein immunoprecipitated by this antibody retained binding activity, and the antibody was used to detect this protein in cells lining bovine liver sinusoids and on the surface of cultured bovine alveolar macrophages. In the human monocytic cell line THP-1, the expression of both acetyl LDL receptor activity and a 220-kDa acetyl LDL binding protein were dramatically induced in parallel after differentiation to a macrophage-like state induced by phorbol ester. The ligand specificity, tissue and cell-type specificity, and coinduction data indicated that this 220-kDa cell-surface binding protein is probably a receptor that mediates acetyl LDL endocytosis. The 220-kDa protein, which was purified 238,000-fold from bovine lung membranes to near homogeneity using monoclonal antibody affinity chromatography, is a trimer of 77-kDa subunits that contain asparagine-linked carbohydrate chains.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The text we found for this paper looks like another document, so the self-check wasn’t run.
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Raza, H., John, A., & Nemmar, A. (2013) · Cellular Physiology and Biochemistry · doi:10.1159/000350087 · Cited by 47,311 · see citing works
The Pathogenesis of Atherosclerosis — An Update
Ross, R. (1986) · New England Journal of Medicine · doi:10.1056/nejm198602203140806 · Cited by 4,885 · see citing works
Goldstein, J. L., Ho, Y. K., Basu, S. K., & Brown, M. S. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.1.333 · Cited by 2,447 · see citing works
LIPOPROTEIN METABOLISM IN THE MACROPHAGE: Implications for Cholesterol Deposition in Atherosclerosis
Brown, M. S., & Goldstein, J. L. (1983) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.52.070183.001255 · Cited by 2,300 · see citing works
Rg, G. (1981) · PubMed · Cited by 1,317 · see citing works
Fogelman, A. M., Shechter, I., Seager, J., Hokom, M. M., CHILD, J. S., & Edwards, P. A. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.4.2214 · Cited by 849 · see citing works
Gething, M.-J. H., McCammon, K. M., & Sambrook, J. (1986) · Cell · doi:10.1016/0092-8674(86)90076-0 · Cited by 846 · see citing works
Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester.
Tsuchiya, S., Kobayashi, Y., Goto, Y., Okumura, H., Nakae, S., Konno, T., & Tada, K. (1982) · PubMed · Cited by 803 · see citing works
Myrvik, Q. N., Leake, E. S., & Fariss, B. L. (1961) · The Journal of Immunology · doi:10.4049/jimmunol.86.2.128 · Cited by 721 · see citing works
Elder, J. H., & Alexander, S. P. H. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.15.4540 · Cited by 688 · see citing works
Tsang, V. C. W., Peralta, J. M., & Simons, A. (1983) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(83)92032-3 · Cited by 672 · see citing works
Reversible accumulation of cholesteryl esters in macrophages incubated with acetylated lipoproteins.
Brown, M. S., Goldstein, J. L., Krieger, M., Ho, Y. K., & Anderson, R. G. W. (1979) · The Journal of Cell Biology · doi:10.1083/jcb.82.3.597 · Cited by 551 · see citing works
Self-citationBrown, M. S., Basu, S. K., Falck, J. R., Ho, Y. K., & Goldstein, J. L. (1980) · Journal of Supramolecular Structure · doi:10.1002/jss.400130107 · Cited by 528 · see citing works
Arterial foam cells with distinctive immunomorphologic and histochemical features of macrophages.
Schaffner, T., Taylor, K., Bartucci, E. J., Fischer-Dzoga, K., Beeson, J. H., Glagov, S. K., & Wissler, R. W. (1980) · PubMed Central · Cited by 475 · see citing works
The role of the monocyte in atherogenesis: II. Migration of foam cells from atherosclerotic lesions.
Gerrity, R. G. (1981) · PubMed · Cited by 459 · see citing works
Kreis, T. E., & Lodish, H. F. (1986) · Cell · doi:10.1016/0092-8674(86)90075-9 · Cited by 423 · see citing works
Nagelkerke, J. F., Barto, K. P., & van Berkel, T. J. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)44160-3 · Cited by 392 · see citing works
Beisiegel, U., Schneider, W. J., Goldstein, J. L., Anderson, R. G., & Brown, M. S. (1981) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)68494-2 · Cited by 357 · see citing works
Adamson, T. C., Fox, R. I., Frisman, D. M., et al. (1983) · The Journal of Immunology · doi:10.4049/jimmunol.130.1.203 · Cited by 311 · see citing works
Visualization of lipoprotein receptors by ligand blotting.
Daniel, T. O., Schneider, W. J., Goldstein, J. L., & Brown, M. S. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32666-8 · Cited by 264 · see citing works
Pitas, R. E., Boyles, J. K., Mahley, R. W., & Bissell, D. M. (1985) · The Journal of Cell Biology · doi:10.1083/jcb.100.1.103 · Cited by 215 · see citing works
Kingsley, D. M., & Krieger, M. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.17.5454 · Cited by 186 · see citing works
Self-citationShechter, I., Fogelman, A. M., Haberland, M. E., Seager, J., Hokom, M. M., & Edwards, P. A. (1981) · Journal of Lipid Research · doi:10.1016/s0022-2275(20)34741-6 · Cited by 183 · see citing works
Schneider, W. J., Goldstein, J. L., & Brown, M. S. (1980) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)70311-1 · Cited by 171 · see citing works
Blomhoff, R., Drevon, C. A., Eskild, W., HELGERUD, P., Norum, K. R., & Berg, T. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)47239-5 · Cited by 126 · see citing works
Krieger, M. (1983) · Cell · doi:10.1016/0092-8674(83)90423-3 · Cited by 94 · see citing works
Self-citationMurine macrophage tumors are a source of a 260,000-dalton acetyl-low density lipoprotein receptor.
Via, D. P., Dresel, H. A., Cheng, S., et al. (1985) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)39619-9 · Cited by 93 · see citing works
Hara, H., Tanishita, H., Yokoyama, S., et al. (1987) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(87)90601-2 · Cited by 87 · see citing works
Mahley, R. W., Weisgraber, K. H., Innerarity, T. L., et al. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.4.1746 · Cited by 70 · see citing works
Kozarsky, K., Brush, H. A., & Krieger, M. (1986) · The Journal of Cell Biology · doi:10.1083/jcb.102.5.1567 · Cited by 58 · see citing works
Self-citationDresel, H. A., Friedrich, E. A., Via, D. P., et al. (1985) · The EMBO Journal · doi:10.1002/j.1460-2075.1985.tb03754.x · Cited by 26 · see citing works
Restoration of LDL Receptor Activity in Mutant Cells by Intercellular Junctional Communication
Hobbie, L. J., Kingsley, D. M., Kozarsky, K., Jackman, R. W., & Krieger, M. (1987) · Science · doi:10.1126/science.3798096 · Cited by 21 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Kinoshita, M., Yokote, K., Arai, H., Iida, M., Ishigaki, Y., Ishibashi, S., Umemoto, S., Egusa, G., Ohmura, H., Okamura, T., et al. (2018) · Journal of Atherosclerosis and Thrombosis · doi:10.5551/jat.gl2017 · Cited by 813 · see citing works
A Null Mutation in Murine CD36 Reveals an Important Role in Fatty Acid and Lipoprotein Metabolism
Febbraio, M., Abumrad, N. A., Hajjar, D. P., Sharma, K., Cheng, W., Pearce, S. F. A., & Silverstein, R. L. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.27.19055 · Cited by 769 · see citing works
Doens, D., & Fernández, P. L. (2014) · Journal of Neuroinflammation · doi:10.1186/1742-2094-11-48 · Cited by 348 · see citing works
Itabe, H., Takeshima, E., Iwasaki, H., Kimura, J., Yoshida, Y., Imanaka, T., & Takano, T. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)36602-4 · Cited by 282 · see citing works
Mato, M., Ookawara, S., Sakamoto, A., Aikawa, E., Ogawa, T., Mitsuhashi, U., Masuzawa, T., Suzuki, H., Honda, M., Yazaki, Y., et al. (1996) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.93.8.3269 · Cited by 187 · see citing works
Self-citation
- Authors
- Kodama, T., Reddy, P., Kishimoto, C., & Krieger, M.
- Affiliations
- Massachusetts Institute of Technology (US)
- ORCID
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1988-12
- Volume
- 85
- Issue
- 23
- Pages
- 9238-9242
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC282714
- OpenAlex
- W2063813098
- Cited by
- 259
- References
- 33
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 4 · 2016: 5 · 2015: 3 · 2014: 6 · 2013: 3 · 2012: 3
- FWCI
- 14.24
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Keywords
- macrophage foam cell formation, monoclonal antibodies, receptor purification, phorbol ester, receptor internalization, asparagine-linked glycosylation, atherosclerosis
- MeSH terms
- Acetylation, Animals, Cattle, Cell Membrane, Chromatography, Affinity, Kinetics, Lipoproteins, LDL, Liver, Macrophages, Membrane Proteins, Receptors, LDL
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review