Localization of glucocorticoid receptor mRNA in the male rat brain by in situ hybridization
Open accessAbstract
The localization and distribution of mRNA encoding the glucocorticoid receptor (GR) was investigated in tissue sections of the adult male rat brain by in situ hybridization and RNA blot analysis. GR mRNA levels were measured by quantitative autoradiography with 35S- and 32P-labeled RNA probes, respectively. Strong labeling was observed within the pyramidal nerve cells of the CA1 and CA2 areas of the hippocampal formation, in the granular cells of the dentate gyrus, in the parvocellular nerve cells of the paraventricular hypothalamic nucleus, and in the cells of the arcuate nucleus, especially the parvocellular part. Moderate labeling of a large number of nerve cells was observed within layers II, III, and VI of the neocortex and in many thalamic nuclei, especially the anterior and ventral nuclear groups as well as several midline nuclei. Within the cerebellar cortex, strong labeling was observed all over the granular layer. In the lower brainstem, strong labeling was found within the entire locus coeruleus and within the mesencephalic raphe nuclei rich in noradrenaline and 5-hydroxytryptamine cell bodies, respectively. A close correlation was found between the distribution of GR mRNA and the distribution of previously described GR immunoreactivity. These studies open the possibility of obtaining additional information on in vivo regulation of GR synthesis and how the brain may alter its sensitivity to circulating glucocorticoids.
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Cites
Single-Step Method of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction
Chomczynski, P., & Sacchi, N. (1987) · Analytical Biochemistry · doi:10.1006/abio.1987.9999 · Cited by 65,240 · see citing works
STEROID RECEPTOR REGULATED TRANSCRIPTION OF SPECIFIC GENES AND GENE NETWORKS
Yamamoto, K. R. (1985) · Annual Review of Genetics · doi:10.1146/annurev.ge.19.120185.001233 · Cited by 1,872 · see citing works
Adrenal steroid receptors and actions in the nervous system
McEwen, B. S., de Kloet, E. R., & Rostène, W. H. (1986) · Physiological Reviews · doi:10.1152/physrev.1986.66.4.1121 · Cited by 1,238 · see citing works
Stress Down-Regulates Corticosterone Receptors in a Site-Specific Manner in the Brain*
Sapolsky, R. M., Krey, L. C., & McEwen, B. S. (1984) · Endocrinology · doi:10.1210/endo-114-1-287 · Cited by 586 · see citing works
Fuxé, K., Wikström, A., Okret, S., Agnati, L. F., Härfstrand, A., Yu, Z., Granholm, L., Zoli, M., Vale, W., & Gustafsson, J.-Α. (1985) · Endocrinology · doi:10.1210/endo-117-5-1803 · Cited by 507 · see citing works
Self-citationGlucocorticoid receptor immunoreactivity in monoaminergic neurons of rat brain.
Härfstrand, A., Fuxé, K., Cintra, A., Agnati, L. F., Zini, I., Wikström, A. C., Okret, S., Yu, Z., Goldstein, M., & Steinbusch, H. W. M. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.24.9779 · Cited by 476 · see citing works
Self-citationDetection of viral sequences of low reiteration frequency by in situ hybridization.
Brahic, M., & Haase, A. T. (1978) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.75.12.6125 · Cited by 454 · see citing works
Reul, J. M. H. M., & de Kloet, E. R. (1986) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(86)90063-4 · Cited by 325 · see citing works
Characterization of a steroid hormone receptor gene and mRNA in wild-type and mutant cells
Miesfeld, R. L., Okret, S., Wikström, A., Wränge, Ö., Gustafsson, J.-Å., & Yamamoto, K. R. (1984) · Nature · doi:10.1038/312779a0 · Cited by 277 · see citing works
Self-citationOkret, S., Poellinger, L., Dong, Y., & Gustafsson, J.-Α. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.16.5899 · Cited by 256 · see citing works
Self-citationTissue-specific regulation of glucocorticoid receptor mRNA by dexamethasone.
Kalinyak, J. E., Dorin, R. I., Hoffman, A. R., & Perlman, A. J. (1987) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)60980-9 · Cited by 228 · see citing works
Corticosterone receptors decline in a site-specific manner in the aged rat brain
Sapolsky, R. M., Krey, L. C., & McEwen, B. S. (1983) · Brain Research · doi:10.1016/0006-8993(83)90024-0 · Cited by 164 · see citing works
Down-regulation of neural corticosterone receptors by corticosterone and dexamethasone
Sapolsky, R. M., & McEwen, B. S. (1985) · Brain Research · doi:10.1016/0006-8993(85)90638-9 · Cited by 106 · see citing works
Glucocorticoid Receptor in Magnocellular Neurosecretory Cells
Kiss, J. Z., EEKELEN, J. A. M. V., Reul, J. M. H. M., Westphal, H. M., & de Kloet, E. R. (1988) · Endocrinology · doi:10.1210/endo-122-2-444 · Cited by 85 · see citing works
Cintra, A., Fuxe, K., Härfstrand, A., Agnati, L. F., Wikström, A.-C., Okret, S., Vale, W., & Gustafsson, J.-Å. (1987) · Neuroscience Letters · doi:10.1016/0304-3940(87)90601-x · Cited by 68 · see citing works
Self-citationFuxé, K., Cintra, A., Härfstrand, A., Agnati, L. F., Kalia, M., Zoli, M., Wikström, A. C., Okret, S., Aronsson, M., & Gustafsson, J.-Å. (1987) · Annals of the New York Academy of Sciences · doi:10.1111/j.1749-6632.1987.tb24974.x · Cited by 65 · see citing works
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Research works printed but not linked: 1
- Fuxe K., Wikström A. C., Okret S., Agnati L. F., Härfstrand A., Yu Z. Y., Granholm L., Zoli M., Vale W., Gustafsson J. A. Mapping of glucocorticoid receptor immunoreactive neurons in the rat tel- and diencephalon using a monoclonal antibody against rat liver glucocorticoid receptor. Endocrinology. 1985 Nov;117(5):1803–1812. doi: 10.1210/endo-117-5-1803. [DOI] [PubMed] [Google Scholar] · DOI
Cited by
Sorted by citations
Brain corticosteroid receptor balance in health and disease
de Kloet, E. R., Vreugdenhil, E., Oitzl, M. S., & Joëls, M. (1998) · Endocrine Reviews · doi:10.1210/edrv.19.3.0331 · Cited by 2,614 · see citing works
Limbic system mechanisms of stress regulation: Hypothalamo-pituitary-adrenocortical axis
Herman, J. P., Ostrander, M. M., Mueller, N. K., & Figueiredo, H. (2005) · Progress in Neuro-Psychopharmacology and Biological Psychiatry · doi:10.1016/j.pnpbp.2005.08.006 · Cited by 1,322 · see citing works
Gonadal steroid hormones and the hypothalamo–pituitary–adrenal axis
Handa, R. J., & Weiser, M. J. (2014) · Frontiers in Neuroendocrinology · doi:10.1016/j.yfrne.2013.11.001 · Cited by 408 · see citing works
Effects of glucocorticoids and norepinephrine on the excitability in the hippocampus
Joëls, M., & de Kloet, E. R. (1989) · Science · doi:10.1126/science.2781292 · Cited by 375 · see citing works
Glucocorticoid and mineralocorticoid receptor mRNA expression in squirrel monkey brain
Patel, P. D., Lopez, J. F., Lyons, D. M., Burke, S., Wallace, M., & Schatzberg, A. F. (2000) · Journal of Psychiatric Research · doi:10.1016/s0022-3956(00)00035-2 · Cited by 252 · see citing works
Hypothalamic-pituitary-adrenocortical axis regulation
Jacobson, L. (2005) · Endocrinology and Metabolism Clinics of North America · doi:10.1016/j.ecl.2005.01.003 · Cited by 248 · see citing works
Hagihara, K., Hirata, S., Osada, T., Hirai, M., & Kato, J. (1992) · Molecular Brain Research · doi:10.1016/0169-328x(92)90179-f · Cited by 134 · see citing works
Flanagan, J. C., Baker, N. L., McRae-Clark, A. L., Brady, K. T., & Moran-Santa Maria, M. M. (2015) · Psychiatry Research · doi:10.1016/j.psychres.2015.07.064 · Cited by 56 · see citing works
Vedder, H., Weiβ, I., Holsboer, F., & Reul, J. M. H. M. (1993) · Brain Research · doi:10.1016/0006-8993(93)91351-r · Cited by 46 · see citing works
Synergistic effects of diet and exercise on hippocampal function in chronically stressed mice
Hutton, C. P., Déry, N., Rosa, E., Lemon, J. A., Rollo, C. D., Boreham, D. R., Fahnestock, M., deCatanzaro, D., Wojtowicz, J. M., & Becker, S. (2015) · Neuroscience · doi:10.1016/j.neuroscience.2015.09.005 · Cited by 41 · see citing works
- Authors
- Aronsson, M., Fuxe, K., Dong, Y., Agnati, L. F., Okret, S., & Gustafsson, J. A.
- Affiliations
- Karolinska Institutet (SE)
- Department of Histology and Neurobiology, Karolinska Institutet, Stockholm, Sweden.
- ORCID
- Okret, S
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1988-12
- Volume
- 85
- Issue
- 23
- Pages
- 9331-9335
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC282733
- OpenAlex
- W1971383287
- Cited by
- 261
- References
- 16
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 4 · 2023: 6 · 2022: 5 · 2021: 6 · 2020: 3 · 2019: 8 · 2018: 7 · 2017: 8 · 2016: 9 · 2015: 8 · 2014: 11 · 2013: 6 · 2012: 11
- FWCI
- 5.31
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Keywords
- glucocorticoid receptor, mRNA localization, brain, hippocampus, dentate gyrus, paraventricular nucleus, arcuate nucleus, neocortex, thalamus, cerebellum, locus coeruleus, raphe nuclei, noradrenergic neurons, glucocorticoid sensitivity
- MeSH terms
- Animals, Autoradiography, Brain, Male, Nucleic Acid Hybridization, Organ Specificity, Rats, Inbred Strains, Receptors, Glucocorticoid, Reference Values, RNA, Messenger, Sulfur Radioisotopes, Immunoblotting, Rats
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