Distribution of cells containing progesterone receptor mRNA in the female rat di- and telencephalon: an in situ hybridization study
Abstract
In an attempt to examine regional synthesis of the progesterone receptor (PR) in the brain, the distribution of mRNA encoding PR was investigated in the female adult rat di- and telencephalon by in situ hybridization using T7 RNA polymerase transcripts of a 320 base pair rat PR cDNA clone. The rat PR cDNA had been partially cloned and sequenced by using the reverse transcription-polymerase chain reaction (RT-PCR) method. The primer set corresponds to a part of the progesterone binding domain of human PR cDNA. Large numbers of strong labeling were observed in the arcuate nucleus, medial preoptic nucleus, and ventrolateral part of the ventromedial nucleus which are relative to sexual behavior. Moderate labeling was found in layers II and IV of the isocortex, in the pyramidal layer of the CA1 and CA3 fields of the hippocampal formation, in the cortical nucleus of the amygdala, in the nucleus of the diagonal band, and in the anterior periventricular nucleus. Weak labeling was found in many other regions. These results were largely in agreement with the distribution of PR previously reported by ligand binding assay and autoradiographic studies. This present in situ hybridization study may provide a useful tool for the analysis of the regional regulation of PR synthesis in the rat brain.
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PR is likely only expressed in neurons in rat cortex.
“and it is likely only expressed in neurons (Lopez and Wagner, 2009; Hagihara et al., 1992).”
From Does puberty mark a transition in sensitive periods for plasticity in the associative neocortex? (2017) · cites this paper as Hagihara et al., 1992
PR is unambiguously expressed in the rat frontal cortex at birth and rises in both males and females until it peaks during the second week of life, after which it significantly declines and is maintained at lower levels into adulthood.
“It is unambiguously expressed in the rat frontal cortex at birth and rises in both males and females until it peaks during the second week of life, after which it significantly declines and is maintained at lower levels into adulthood (Hagihara et al., 1992; Kato and Onouchi, 1981; Kato and Onouchi, 1983; Kato et al., 1984; Lopez and Wagner, 2009; Shughrue et al., 1991; Shughrue et al., 1992).”
From Does puberty mark a transition in sensitive periods for plasticity in the associative neocortex? (2017) · cites this paper as Hagihara et al., 1992
A general theme in the literature is that PR binding decreases before weaning and is maintained at mid to low levels into adulthood; this general pattern emerges in mRNA experiments as well.
“However, a general theme in the literature is that PR binding decreases before weaning and is maintained at mid to low levels into adulthood (Kato and Onouchi, 1981; Kato and Onouchi, 1983; Kato et al., 1984; Parsons et al., 1982); this general pattern emerges in mRNA experiments as well (Hagihara et al., 1992; Intlekofer and Petersen, 2011).”
From Does puberty mark a transition in sensitive periods for plasticity in the associative neocortex? (2017) · cites this paper as Hagihara et al., 1992
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- Authors
- Hagihara, K., Hirata, S., Osada, T., Hirai, M., & Kato, J.
- Affiliations
- University of Yamanashi Hospital (JP)
- University of Yamanashi (JP)
- Corresponding author
- Kato, Junzo
- Venue
- Molecular Brain Research
- Publisher
- Elsevier BV
- Published
- 1992-07
- Volume
- 14
- Issue
- 3
- Pages
- 239-249
- ISSN
- 0169-328X (ISSN-L)
- OpenAlex
- W1988471030
- Cited by
- 134
- References
- 53
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 4 · 2022: 1 · 2021: 3 · 2020: 1 · 2019: 2 · 2018: 4 · 2017: 4 · 2016: 3 · 2014: 5 · 2013: 3 · 2012: 4
- FWCI
- 1.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Keywords
- progesterone receptor, arcuate nucleus, medial preoptic nucleus, ventromedial hypothalamus, hippocampus, amygdala, diagonal band, periventricular nucleus, sexual behavior, regional gene expression
- MeSH terms
- Animals, Cloning, Molecular, Diencephalon, Estrus, Female, Neurons, Nucleic Acid Hybridization, Organ Specificity, Receptors, Progesterone, RNA, Messenger, Telencephalon, RNA Probes, Polymerase Chain Reaction, Rats, Wistar, Rats
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