The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.
Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.
Open accessAbstract
Picomole amounts of oligodeoxynucleotides [polydeoxyadenylic acids, (dA)40-60] were baseline resolved and analyzed in less than 8 min by high-performance capillary electrophoresis with polyacrylamide gels. In addition, fast analysis of a crude 70-mer oligodeoxynucleotide and a slab gel-purified 99-mer oligodeoxynucleotide was accomplished, demonstrating the ability of high-performance capillary electrophoresis to characterize rapidly synthesized oligonucleotides. Besides analytical separations, 800 ng of a primer (20-mer) was isolated in less than 20 min. The purified species was collected in water and subsequently used as a probe in a standard dot-blot analysis. The use of high-performance capillary electrophoresis for the analysis and purification of a variety of biopolymers is simple, rapid, and has the potential for automation.
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Cites
A new method for sequencing DNA.
Maxam, A. M., & Gilbert, W. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.2.560 · Cited by 7,922
Capillary Zone Electrophoresis
Jorgenson, J. W., & Lukacs, K. D. (1983) · Science · doi:10.1126/science.6623076 · Cited by 1,289
Hjertén, S. (1967) · Chromatographic Reviews · doi:10.1016/0009-5907(67)80003-6 · Cited by 728
Cohen, A. S., & Karger, B. L. (1987) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)85026-3 · Cited by 378
Self-citationTotal synthesis of a human leukocyte interferon gene
Edge, M. D., Greene, A. R., Heathcliffe, G. R., Meacock, P. A., Schuch, W., Scanlon, D. B., Atkinson, T. C., Newton, C. R., & Markham, A. F. (1981) · Nature · doi:10.1038/292756a0 · Cited by 159
SERPERSU, E. H., Shortle, D., & Mildvan, A. S. (1986) · Biochemistry · doi:10.1021/bi00349a011 · Cited by 120
Cited by
Heiger, D. N., Cohen, A. S., & Karger, B. L. (1990) · Journal of Chromatography A · doi:10.1016/s0021-9673(01)90202-x · Cited by 397
Self-citationCapillary gel electrophoresis for rapid, high resolution DNA sequencing
Swerdlow, H., & Gesteland, R. (1990) · Nucleic Acids Research · doi:10.1093/nar/18.6.1415 · Cited by 360
High-performance capillary electrophoresis
Karger, B. L. (1989) · Nature · doi:10.1038/339641a0 · Cited by 318
Self-citationButler, J. M., Buel, E., Crivellente, F., & McCord, B. R. (2004) · ELECTROPHORESIS · doi:10.1002/elps.200305822 · Cited by 276
High-speed separations of DNA sequencing reactions by capillary electrophoresis
Drossman, H., Luckey, J. A., Kostichka, A. J., D'Cunha, J., & Smith, L. M. (1990) · Analytical Chemistry · doi:10.1021/ac00208a003 · Cited by 265
Metadata
- Authors
- Cohen, A. S., Najarian, D. R., Paulus, A., Guttman, A., Smith, J. A., & Karger, B. L.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1988-12
- Volume
- 85
- Issue
- 24
- Pages
- 9660-9663
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1990580218
- Cited by
- 318
- References
- 6
- FWCI
- 25.27
- Type
- journal article
- Language
- en
- Open access
- green
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