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Cited by 318

The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.

Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis.

Open access
Cohen, A S, Najarian, D R, Paulus, A, Guttman, A, Smith, J A, Karger, B L·1988·Proceedings of the National Academy of Sciences

Abstract

Picomole amounts of oligodeoxynucleotides [polydeoxyadenylic acids, (dA)40-60] were baseline resolved and analyzed in less than 8 min by high-performance capillary electrophoresis with polyacrylamide gels. In addition, fast analysis of a crude 70-mer oligodeoxynucleotide and a slab gel-purified 99-mer oligodeoxynucleotide was accomplished, demonstrating the ability of high-performance capillary electrophoresis to characterize rapidly synthesized oligonucleotides. Besides analytical separations, 800 ng of a primer (20-mer) was isolated in less than 20 min. The purified species was collected in water and subsequently used as a probe in a standard dot-blot analysis. The use of high-performance capillary electrophoresis for the analysis and purification of a variety of biopolymers is simple, rapid, and has the potential for automation.

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Metadata

Authors
Cohen, A. S., Najarian, D. R., Paulus, A., Guttman, A., Smith, J. A., & Karger, B. L.
Venue
Proceedings of the National Academy of Sciences
Published
1988-12
Volume
85
Issue
24
Pages
9660-9663
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W1990580218
Cited by
318
References
6
FWCI
25.27
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis. https://app.cochat.ai/papers/10.1073/pnas.85.24.9660
The paper
Cohen, A. S., Najarian, D. R., Paulus, A., Guttman, A., Smith, J. A., & Karger, B. L. (1988). Rapid separation and purification of oligonucleotides by high-performance capillary gel electrophoresis. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.85.24.9660

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