The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
A phospholipase D specific for the phosphatidylinositol anchor of cell-surface proteins is abundant in plasma.
Open accessAbstract
An enzyme activity capable of degrading the glycosyl-phosphatidylinositol membrane anchor of cell-surface proteins has previously been reported in a number of mammalian tissues. The experiments reported here demonstrate that this anchor-degrading activity is also abundant in mammalian plasma. The activity was inhibited by EGTA or 1,10-phenanthroline. It was capable of removing the anchor from alkaline phosphatase, 5'-nucleotidase, and variant surface glycoprotein but had little or not activity toward phosphatidylinositol or phosphatidylcholine. Phosphatidic acid was the only 3H-labeled product when this enzyme hydrolyzed [3H]myristate-labeled variant surface glycoprotein. It could be distinguished from the Ca2+-dependent inositol phospholipid-specific phospholipase C activity in several rat tissues on the basis of its molecular size and its sensitivity to 1,10-phenanthroline. The data therefore suggest that this activity is due to a phospholipase D with specificity for glycosyl-phosphatidylinositol structures. Although the precise physiological function of this anchor-specific phospholipase D remains to be determined, these findings indicate that it could play an important role in regulating the expression and release of cell-surface proteins in vivo.
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Cites
The Metabolism of Phosphoinositide-Derived Messenger Molecules
Majerus, P. W., Connolly, T. M., Deckmyn, H., Ross, T. S., Bross, T. E., Ishii, H., Bansal, V. S., & Wilson, D. B. (1986) · Science · doi:10.1126/science.3024320 · Cited by 715
Biochemistry of the glycosyl-phosphatidylinositol membrane protein anchors
Low, M. G. (1987) · Biochemical Journal · doi:10.1042/bj2440001 · Cited by 592
Self-citationGrowth factor-like action of phosphatidic acid
Moolenaar, W. H., Kruijer, W., Tilly, B. C., Verlaan, I., Bierman, A. J., & de Laat, S. W. (1986) · Nature · doi:10.1038/323171a0 · Cited by 513
Role of phosphatidylinositol in attachment of alkaline phosphatase to membranes
Low, M. G., & Zilversmit, D. B. (1980) · Biochemistry · doi:10.1021/bi00558a004 · Cited by 256
Self-citationA Glycan-Phosphatidylinositol-Specific Phospholipase D in Human Serum
Davitz, M. A., Hereld, D., Shak, S., Krakow, J., Englund, P. T., & Nussenzweig, V. (1987) · Science · doi:10.1126/science.2443973 · Cited by 250
Bülow, R., & Overath, P. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)67328-4 · Cited by 144
Fox, J. A., Duszenko, M., Ferguson, M. A. J., Low, M. G., & Cross, G. (1986) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)66784-5 · Cited by 142
Self-citation
Cited by
Horowitz, A. M., Fan, X., Bieri, G., Smith, L. K., Sanchez-Diaz, C. I., Schroer, A. B., Gontier, G., Casaletto, K. B., Kramer, J. H., Williams, K. E., et al. (2020) · Science · doi:10.1126/science.aaw2622 · Cited by 496
Biosynthesis and biology of mammalian GPI-anchored proteins
Kinoshita, T. (2020) · Open Biology · doi:10.1098/rsob.190290 · Cited by 341
Metadata
- Authors
- Low, M. G., & Prasad, A. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1988-02
- Volume
- 85
- Issue
- 4
- Pages
- 980-984
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2011603959
- Cited by
- 261
- References
- 7
- FWCI
- 11.44
- Type
- journal article
- Language
- en
- Open access
- green
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