Cloning and expression of the gene cluster encoding key proteins involved in acetyl-CoA synthesis in Clostridium thermoaceticum: CO dehydrogenase, the corrinoid/Fe-S protein, and methyltransferase

Roberts, D L; James-Hagstrom, J E; Garvin, D K; Gorst, C M; Runquist, J A; Baur, J R; Haase, F C; Ragsdale, S W

doi:10.1073/pnas.86.1.32

Cited by 56Metalloenzymes and iron-sulfur proteinsMicrobial metabolism and enzyme functionAnaerobic Digestion and Biogas Production

Cloning and expression of the gene cluster encoding key proteins involved in acetyl-CoA synthesis in Clostridium thermoaceticum: CO dehydrogenase, the corrinoid/Fe-S protein, and methyltransferase

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Roberts, D L, James-Hagstrom, J E, Garvin, D K, Gorst, C M, Runquist, J A, Baur, J R, Haase, F C, Ragsdale, S W·1989·Proceedings of the National Academy of Sciences

DOI 10.1073/pnas.86.1.32PMC PMC286397

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Abstract

Acetogenic bacteria fix CO or CO2 by a pathway of autotrophic growth called the acetyl-CoA (or Wood) pathway. Key enzymes in the pathway are a methyltransferase, a corrinoid/Fe-S protein, a disulfide reductase, and a carbon monoxide dehydrogenase. This manuscript describes the isolation of the genes that code for the methyltransferase, the two subunits of the corrinoid/Fe-S protein, and the two subunits of carbon monoxide dehydrogenase. These five genes were found to be clustered within an approximately 10-kilobase segment on the Clostridium thermoaceticum genome. The proteins were expressed at up to 5-10% of Escherichia coli cell protein, and isopropyl beta-D-thiogalactopyranoside had no effect on the levels of expression, implying that the C. thermoaceticum inserts contained transcriptional and translational signals that were recognized by E. coli. The methyltransferase is expressed in E. coli in a fully active dimeric form with a specific activity and heat stability similar to the enzyme expressed in C. thermoaceticum. However, both the corrinoid/Fe-S protein and carbon dioxide dehydrogenase, although expressed in high amounts and with identical subunit molecular weights in E. coli, are inactive and less heat stable than are the native enzymes from C. thermoaceticum.

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The reductive acetyl coenzyme A pathway: sequence and heterologous expression of active methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase from Clostridium thermoaceticum
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Cloning, Sequencing, and Expressions of Genes Encoding Enzymes of the Autotrophic Acetyl-CoA Pathway in the Acetogen Clostridium thermoaceticum
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Levels of enzymes involved in the synthesis of acetate from CO2 in Clostridium thermoautotrophicum
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Acetyl-coenzyme A synthesis from methyltetrahydrofolate, CO, and coenzyme A by enzymes purified from Clostridium thermoaceticum: attainment of in vivo rates and identification of rate-limiting steps
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Authors: Roberts, D. L., James-Hagstrom, J. E., Garvin, D. K., Gorst, C. M., Runquist, J. A., Baur, J. R., Haase, F. C., & Ragsdale, S. W.
Affiliations: University of Wisconsin–Milwaukee (US)
ORCID: Ragsdale, S W
Venue: Proceedings of the National Academy of Sciences
Publisher: National Academy of Sciences
Published: 1989-01
Volume: 86
Issue: 1
Pages: 32-36
ISSN: 0027-8424 (ISSN-L)
1091-6490
DOI: 10.1073/pnas.86.1.32
PMC: PMC286397
OpenAlex: W2022642835
Cited by: 56
References: 28
Citations by year: 2024: 1 · 2023: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 1 · 2016: 2 · 2015: 3 · 2013: 1 · 2012: 1
FWCI: 7.00
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Locations: Proceedings of the National Academy of Sciences (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: National Institutes of Health (5r29gm039451-02)
National Institute of General Medical Sciences (1-R29-GM39451)
Topics: Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Metalloenzymes and iron-sulfur proteins
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
Physical Sciences › Engineering › Building and Construction › Anaerobic Digestion and Biogas Production
Keywords: Clostridium thermoaceticum, acetyl-CoA biosynthesis, acetogenic bacteria, gene clusters, carbon monoxide dehydrogenase, methyltransferase, heterologous expression in Escherichia coli, enzyme thermostability, enzyme activity
MeSH terms: Acetyl Coenzyme A, Aldehyde Oxidoreductases, Amino Acid Sequence, Clostridium, DNA, Bacterial, Genes, Genes, Bacterial, Multigene Family, Iron-Sulfur Proteins, Metalloproteins, Methyltransferases, Molecular Sequence Data, Multienzyme Complexes, Nucleic Acid Hybridization, Plasmids, Vitamin B 12, Restriction Mapping, Corrinoids

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Cloning and expression of the gene cluster encoding key proteins involved in acetyl-CoA synthesis in Clostridium thermoaceticum: CO dehydrogenase, the corrinoid/Fe-S protein, and methyltransferase

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