Metabolic engineering strategies to enable microbial utilization of C1 feedstocks
Abstract
One-carbon (C1) substrates are preferred feedstocks for the biomanufacturing industry and have recently gained attention owing to their natural abundance, low production cost and availability as industrial by-products. However, native pathways to utilize these substrates are absent in most biotechnologically relevant microorganisms. Recent advances in synthetic biology, genome engineering and laboratory evolution are enabling the first steps towards the creation of synthetic C1-utilizing microorganisms. Here, we briefly review the native metabolism of methane, methanol, CO 2, CO and formate, and how these C1-utilizing pathways can be engineered into heterologous hosts. In addition, this review analyses the potential, the challenges and the perspectives of C1-based biomanufacturing.
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Metabolic engineering can enable microbial utilization of CO2, CH4, and other C1 feedstocks for production of microbial proteins rich in essential amino acids.
“Other novel biotechnology strategies include metabolic engineering to enable microbial utilization of using CO2, CH4, and other C1 feedstocks for the production of microbial proteins rich in essential amino acids.139,140”
From Technologies and perspectives for achieving carbon neutrality (2021) · cites this paper as [140]
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Technologies and perspectives for achieving carbon neutrality
Wang, F., Harindintwali, J. D., Yuan, Z., Wang, M., Wang, F., Li, S., Yin, Z., Huang, L., Fu, Y., Li, L., et al. (2021) · The Innovation · doi:10.1016/j.xinn.2021.100180 · Cited by 1,330 · see citing works
Self-citation
- Authors
- Jiang, W., Hernández Villamor, D., Peng, H., Chen, J., Liu, L., Haritos, V., & Ledesma-Amaro, R.
- Affiliations
- Imperial College London (GB)
- Jiangnan University (CN)
- Monash University (AU)
- ORCID
- Venue
- Nature Chemical Biology
- Published
- 2021-07-26
- Volume
- 17
- Issue
- 8
- Pages
- 845-855
- ISSN
- 1552-4450 (ISSN-L)
- 1552-4469
- OpenAlex
- W3185130928
- Cited by
- 216
- References
- 121
- Citations by year
- 2026: 38 · 2025: 64 · 2024: 39 · 2023: 36 · 2022: 31 · 2021: 8
- FWCI
- 2.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Nature Chemical Biology (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Spiral (Imperial College London) (submittedVersion)
- Funders
- Biotechnology and Biological Sciences Research Council (BB/R01602X/1, BB/T011408/1, BB/T013176/1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
- Physical Sciences › Chemical Engineering › Catalysis › Catalysts for Methane Reforming
- Keywords
- C1 feedstock, metabolic engineering, synthetic biology, methane metabolism, methanol metabolism, CO2 fixation, carbon monoxide metabolism, formate metabolism, heterologous expression, genome editing, experimental evolution
- MeSH terms
- Methanol, Bacteria, Carbon Dioxide, Carbon Monoxide, Formates, Fungi, Methane, Metabolic Engineering
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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