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Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin.
Open accessAbstract
The capacity of Shigella to spread within the cytosol of infected epithelial cells and to infect adjacent cells is critical for the development of infection foci, which lead to mucosal abscesses. Shigella is a nonmotile microorganism that appears to utilize host cell microfilaments to generate intra- as well as intercellular movements, since this movement was inhibited by cytochalasin D and involvement of F-actin was demonstrated by direct labeling of infected cells with the specific dye N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)phallacidin. Such movements led to the formation of extracellular protrusions, which may explain cell to cell spread. icsA, a locus necessary for intra- and intercellular spread, was identified on the Shigella flexneri virulence plasmid pWR100. This locus was cloned and shown to express a 120-kDa outer membrane protein, which plays an important role in the interactions established between host cell microfilaments and the bacterial surface, thus leading to intracellular movement.
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Cited by
L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein
Kocks, C., Gouin, E., Tabouret, M., Berche, P., Ohayon, H., & Cossart, P. (1992) · Cell · doi:10.1016/0092-8674(92)90188-i · Cited by 817
The actin cytoskeleton and actin-based motility
Svitkina, T. (2018) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a018267 · Cited by 761
Exploitation of Mammalian Host Cell Functions by Bacterial Pathogens
Finlay, B. B., & Cossart, P. (1997) · Science · doi:10.1126/science.276.5313.718 · Cited by 746
Domann, E., Wehland, J., Rohde, M., Pistor, S., Hartl, M., Goebel, W., Leimeister-Wächter, M., Wuenscher, M., & Chakraborty, T. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05252.x · Cited by 411
Snapper, S. B., Takeshima, F., Antón, I., Liu, C.-H., Thomas, S. M., Nguyen, D., Dudley, D., Fraser, H., Purich, D., Lopez-Ilasaca, M., et al. (2001) · Nature Cell Biology · doi:10.1038/ncb1001-897 · Cited by 332
Metadata
- Authors
- Bernardini, M. L., Mounier, J., d'Hauteville, H., Coquis-Rondon, M., & Sansonetti, P. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-05
- Volume
- 86
- Issue
- 10
- Pages
- 3867-3871
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1988282769
- Cited by
- 722
- References
- 0
- FWCI
- 14.37
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin. https://app.cochat.ai/papers/10.1073/pnas.86.10.3867
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- Bernardini, M. L., Mounier, J., d'Hauteville, H., Coquis-Rondon, M., & Sansonetti, P. J. (1989). Identification of icsA, a plasmid locus of Shigella flexneri that governs bacterial intra- and intercellular spread through interaction with F-actin. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.10.3867
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