Cited by 817Listeria monocytogenes in Food SafetyBacterial Genetics and BiotechnologyEscherichia coli research studies

L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein

Kocks, C., Gouin, E., Tabouret, M., Berche, P., Ohayon, H., Cossart, P.·1992·Cell

Abstract

The intracellular pathogenic bacterium L. monocytogenes can spread directly from cell to cell without leaving the cytoplasm. The mechanism of this movement, generated through bacterially induced actin polymerization, is not understood. By analyzing an avirulent Tn917-lac mutant defective for actin polymerization, we have identified a bacterial component involved in this process. The transposon had inserted in actA, the second gene of an operon. Gene disruption of downstream genes and transformation of the mutant strain with actA showed that the actA gene encodes a surface protein necessary for bacterially induced actin assembly. Our results indicate that it is a 610 amino acid protein with an apparent molecular weight of 90 kd.

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Crossref · 10.1016/0092-8674(92)90188-i

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  • Insertion of surface proteins into the plasma membrane via an alpha-helical membrane anchor structure occurs for ActA of L. monocytogenes.

    “(iv) Insertion of surface proteins into the plasma membrane via an alpha-helical membrane anchor structure (ActA of L. monocytogenes) [11].”

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Authors
Kocks, C., Gouin, E., Tabouret, M., Berche, P., Ohayon, H., & Cossart, P.
Affiliations
  • Institut Pasteur (FR)
  • Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
  • Hôpital Necker-Enfants Malades (FR)
Venue
Cell
Publisher
Elsevier BV
Published
1992-02
Volume
68
Issue
3
Pages
521-531
ISSN
0092-8674 (ISSN-L)
OpenAlex
W2094070743
Cited by
817
References
50
Citations by year
2026: 11 · 2025: 17 · 2024: 14 · 2023: 19 · 2022: 20 · 2021: 18 · 2020: 26 · 2019: 23 · 2018: 20 · 2017: 20 · 2016: 32 · 2015: 17 · 2014: 32 · 2013: 21 · 2012: 18
FWCI
35.56
Type
journal article
Language
en
Open access
closed
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Listeria monocytogenes in Food Safety
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
Keywords
Listeria monocytogenes, actA gene, actin polymerization, bacterial surface proteins, cell-to-cell transmission, intracellular motility, avirulent mutants, gene disruption, operon
MeSH terms
Actins, Amino Acid Sequence, Animals, Bacterial Proteins, Base Sequence, Cloning, Molecular, Genes, Bacterial, Listeria monocytogenes, Membrane Proteins, Molecular Sequence Data, Oligonucleotides, Endopeptidases, Phospholipases, Virulence, 3T3 Cells, Mice

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