L. monocytogenes-induced actin assembly requires the actA gene product, a surface protein
Abstract
The intracellular pathogenic bacterium L. monocytogenes can spread directly from cell to cell without leaving the cytoplasm. The mechanism of this movement, generated through bacterially induced actin polymerization, is not understood. By analyzing an avirulent Tn917-lac mutant defective for actin polymerization, we have identified a bacterial component involved in this process. The transposon had inserted in actA, the second gene of an operon. Gene disruption of downstream genes and transformation of the mutant strain with actA showed that the actA gene encodes a surface protein necessary for bacterially induced actin assembly. Our results indicate that it is a 610 amino acid protein with an apparent molecular weight of 90 kd.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Insertion of surface proteins into the plasma membrane via an alpha-helical membrane anchor structure occurs for ActA of L. monocytogenes.
“(iv) Insertion of surface proteins into the plasma membrane via an alpha-helical membrane anchor structure (ActA of L. monocytogenes) [11].”
From Protein sorting to the cell wall envelope of Gram-positive bacteria (2004) · cites this paper as [11]
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Improved tools for biological sequence comparison.
Pearson, W. R., & Lipman, D. J. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.8.2444 · Cited by 11,227 · see citing works
Rapid and sensitive protein similarity searches
Lipman, D. J., & Pearson, W. R. (1985) · Science · doi:10.1126/science.2983426 · Cited by 4,033 · see citing works
Intracellular protein topogenesis
Blobel, G. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.3.1496 · Cited by 1,393 · see citing works
Heukeshoven, J., & Dernick, R. (1985) · Electrophoresis · doi:10.1002/elps.1150060302 · Cited by 1,348 · see citing works
Tilney, L. G., & Portnoy, D. A. (1989) · The Journal of Cell Biology · doi:10.1083/jcb.109.4.1597 · Cited by 1,266 · see citing works
Role of hemolysin for the intracellular growth of Listeria monocytogenes.
Portnoy, P. S., Jacks, P. S., & Hinrichs, D. J. (1988) · The Journal of Experimental Medicine · doi:10.1084/jem.167.4.1459 · Cited by 872 · see citing works
Fluorescent phallotoxin, a tool for the visualization of cellular actin.
Wulf, E., Deboben, A., Bautz, F. A., Faulstich, H., & Wieland, T. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.9.4498 · Cited by 789 · see citing works
Listeria monocytogenes and listeric infections
Gray, M. L., & Killinger, A. H. (1966) · Bacteriological Reviews · doi:10.1128/br.30.2.309-382.1966 · Cited by 747 · see citing works
Bernardini, M. L., Mounier, J., D'Hauteville, H., Coquis-Rondon, M., & Sansonetti, P. J. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.10.3867 · Cited by 722 · see citing works
Gaillard, J., Berche, P., Mounier, J., Richard, S., & Sansonetti, P. J. (1987) · Infection and Immunity · doi:10.1128/iai.55.11.2822-2829.1987 · Cited by 715 · see citing works
Self-citationComparative biology of intracellular parasitism
Moulder, J. W. (1985) · Microbiological Reviews · doi:10.1128/mr.49.3.298-337.1985 · Cited by 437 · see citing works
Vázquez-Boland, J. A., Kocks, C., Dramsi, S., Ohayon, H., Geoffroy, C., Mengaud, J., & Cossart, P. (1992) · Infection and Immunity · doi:10.1128/iai.60.1.219-230.1992 · Cited by 395 · see citing works
Self-citationListeria monocytogenes and listeric infections.
Gray, M. L., & Killinger, A. H. (1966) · Bacteriological Reviews · doi:10.1128/mmbr.30.2.309-382.1966 · Cited by 388 · see citing works
Lisanti, M. P., Sargiacomo, M., Graeve, L., Saltiel, A. R., & Rodriguez-Boulan, E. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.24.9557 · Cited by 382 · see citing works
Identification of an actin-binding protein from Dictyostelium as elongation factor 1a
Yang, F., Demma, M. J., Warren, V. A., et al. (1990) · Nature · doi:10.1038/347494a0 · Cited by 346 · see citing works
Dabiri, G. A., Sanger, J. M., Portnoy, P. S., & Southwick, F. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.16.6068 · Cited by 318 · see citing works
Shaw, J. H., & Clewell, D. B. (1985) · Journal of Bacteriology · doi:10.1128/jb.164.2.782-796.1985 · Cited by 318 · see citing works
Makino, S., Sasakawa, C., Kamata, K., et al. (1986) · Cell · doi:10.1016/0092-8674(86)90880-9 · Cited by 309 · see citing works
Mounier, J., Ryter, A., Coquis-Rondon, M., et al. (1990) · Infection and Immunity · doi:10.1128/iai.58.4.1048-1058.1990 · Cited by 299 · see citing works
Hartwig, J. H., & Kwiatkowski, D. J. (1991) · Current Opinion in Cell Biology · doi:10.1016/0955-0674(91)90170-4 · Cited by 289 · see citing works
Pleiotropic control of Listeria monocytogenes virulence factors by a gene that is autoregulated
Mengaud, J., Dramsi, S., Gouin, E., Vázquez-Boland, J. A., Milon, G., & Cossart, P. (1991) · Molecular Microbiology · doi:10.1111/j.1365-2958.1991.tb02158.x · Cited by 272 · see citing works
Self-citationSullivan, M. A., Yasbin, R. E., & Young, F. E. (1984) · Gene · doi:10.1016/0378-1119(84)90161-6 · Cited by 258 · see citing works
Sun, A., Camilli, A., & Portnoy, D. A. (1990) · Infection and Immunity · doi:10.1128/iai.58.11.3770-3778.1990 · Cited by 252 · see citing works
Plaque formation by virulent Shigella flexneri
Oaks, E. V., Wingfield, M. E., & Formal, S. B. (1985) · Infection and Immunity · doi:10.1128/iai.48.1.124-129.1985 · Cited by 249 · see citing works
Lett, M. C., Sasakawa, C., Okada, N., et al. (1989) · Journal of Bacteriology · doi:10.1128/jb.171.1.353-359.1989 · Cited by 241 · see citing works
Comparative biology of intracellular parasitism.
Moulder, J. W. (1985) · Microbiological Reviews · doi:10.1128/mmbr.49.3.298-337.1985 · Cited by 204 · see citing works
Michel, E., Reich, K. A., Favier, R., Berche, P., & Cossart, P. (1990) · Molecular Microbiology · doi:10.1111/j.1365-2958.1990.tb00578.x · Cited by 193 · see citing works
Self-citationCloning and Expression of a Smooth Muscle Caldesmon
Bryan, J., Imai, M., Lee, R., et al. (1989) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)80081-3 · Cited by 192 · see citing works
Vandekerckhove, J. S. (1990) · Current Opinion in Cell Biology · doi:10.1016/s0955-0674(05)80029-8 · Cited by 162 · see citing works
Domann, E., Leimeister-Wächter, M., Goebel, W., et al. (1991) · Infection and Immunity · doi:10.1128/iai.59.1.65-72.1991 · Cited by 135 · see citing works
Actin filament nucleation by the bacterial pathogen, Listeria monocytogenes.
Tilney, L. G., Connelly, P. S., & Portnoy, D. A. (1990) · The Journal of Cell Biology · doi:10.1083/jcb.111.6.2979 · Cited by 133 · see citing works
West, R. R., Tenbarge, K. M., & Olmsted, J. B. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)54720-7 · Cited by 127 · see citing works
Mengaud, J., Geoffroy, C., & Cossart, P. (1991) · Infection and Immunity · doi:10.1128/iai.59.3.1043-1049.1991 · Cited by 125 · see citing works
Self-citationListeria monocytogenes. A model system for the molecular study of intracellular parasitism.
Cossart, P., & Mengaud, J. (1989) · PubMed · Cited by 118 · see citing works
Self-citationMengaud, J., Vicente, M. F., & Cossart, P. (1989) · Infection and Immunity · doi:10.1128/iai.57.12.3695-3701.1989 · Cited by 112 · see citing works
Self-citationKadurugamuwa, J. L., Rohde, M., Wehland, J., et al. (1991) · Infection and Immunity · doi:10.1128/iai.59.10.3463-3471.1991 · Cited by 108 · see citing works
Pál, T., Newland, J. W., Tall, B. D., et al. (1989) · Infection and Immunity · doi:10.1128/iai.57.2.477-486.1989 · Cited by 95 · see citing works
Havell, E. A. (1986) · Infection and Immunity · doi:10.1128/iai.54.3.787-792.1986 · Cited by 85 · see citing works
cDNA-derived sequence of chicken embryo vinculin.
Coutu, M. D., & Craig, S. W. (1988) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.85.22.8535 · Cited by 83 · see citing works
Tn10-derived transposons active in Bacillus subtilis
Petit, M., Bruand, C., Jannière, L., et al. (1990) · Journal of Bacteriology · doi:10.1128/jb.172.12.6736-6740.1990 · Cited by 79 · see citing works
Cloning and expression of theListeria monocytogeneshaemolysin inEscherichia coli
Vicente, M. F., Baquero, F., & Pérez-DÃaz, J. C. (1985) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1985.tb00988.x · Cited by 78 · see citing works
Kuhn, M., Prévost, M., Mounier, J., et al. (1990) · Infection and Immunity · doi:10.1128/iai.58.11.3477-3486.1990 · Cited by 66 · see citing works
Junctional plasma membrane domains isolated from aggregating Dictyostelium discoideum amebae.
Ingalls, H. M., Goodloe-Holland, C. M., & Luna, E. J. (1986) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.83.13.4779 · Cited by 54 · see citing works
Rycke, J. D., Phan‐Thanh, L., & Bernard, S. (1989) · Journal of Clinical Microbiology · doi:10.1128/jcm.27.5.983-988.1989 · Cited by 52 · see citing works
Isolation and characterization of a vinculin cDNA from chick-embryo fibroblasts
Price, G. J., Jones, P. M., Davison, M. D., et al. (1987) · Biochemical Journal · doi:10.1042/bj2450595 · Cited by 50 · see citing works
The complete cDNA sequence of mouse elongation factor 1 alpha (EF 1 alpha) mRNA
Lu, X., & Werner, D. (1989) · Nucleic Acids Research · doi:10.1093/nar/17.1.442 · Cited by 48 · see citing works
Rácz, P., Tenner, K., & Szivessy, K. (1970) · PubMed · Cited by 47 · see citing works
Production of Opacity in Egg-Yolk Medium by Listeria monocytogenes
Füzi, M., & Pillis, I. (1962) · Nature · doi:10.1038/196195a0 · Cited by 26 · see citing works
Genetic transformation between strains of Listeria monocytogenes
Lucas, R. D., & Levin, R. E. (1989) · Letters in Applied Microbiology · doi:10.1111/j.1472-765x.1989.tb00329.x · Cited by 3 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Pathogenicity Islands in BacterialPathogenesis
Schmidt, H., & Hensel, M. (2004) · Clinical Microbiology Reviews · doi:10.1128/cmr.17.1.14-56.2004 · Cited by 776 · see citing works
Interactions of commensal and pathogenic microorganisms with the intestinal mucosal barrier
Martens, E. C., Neumann, M., & Desai, M. S. (2018) · Nature Reviews Microbiology · doi:10.1038/s41579-018-0036-x · Cited by 711 · see citing works
Cell wall sorting signals in surface proteins of gram‐positive bacteria.
Schneewind, O., Mihaylova‐Petkov, D., & Model, P. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb06169.x · Cited by 426 · see citing works
Protein sorting to the cell wall envelope of Gram-positive bacteria
Ton-That, H., Marraffini, L. A., & Schneewind, O. (2004) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2004.04.014 · Cited by 266 · see citing works
Jonquières, R., Bierne, H., Fiedler, F., Gounon, P., & Cossart, P. (1999) · Molecular Microbiology · doi:10.1046/j.1365-2958.1999.01652.x · Cited by 251 · see citing works
Self-citationRobbins, J. R., Skrzypczynska, K. M., Zeldovich, V. B., Kapidzic, M., & Bakardjiev, A. I. (2010) · PLoS Pathogens · doi:10.1371/journal.ppat.1000732 · Cited by 212 · see citing works
Sortase, a universal target for therapeutic agents against Gram-positive bacteria?
Cossart, P., & Jonquières, R. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.97.10.5013 · Cited by 203 · see citing works
Self-citationHussain, M., Becker, K., von Eiff, C., Schrenzel, J., Peters, G., & Herrmann, M. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.23.6778-6786.2001 · Cited by 149 · see citing works
The Cell Biology of Infection by Intracellular Bacterial Pathogens
Theriot, J. A. (1995) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cb.11.110195.001241 · Cited by 122 · see citing works
Sortase B, a New Class of Sortase in<i>Listeria monocytogenes</i>
Bierne, H., Garandeau, C., Pucciarelli, M. G., Sabet, C., Newton, S., Garcia-del Portillo, F., Cossart, P., & Charbit, A. (2004) · Journal of Bacteriology · doi:10.1128/jb.186.7.1972-1982.2004 · Cited by 99 · see citing works
Self-citationFisher, M., Huang, Y.-S., Li, X., McIver, K. S., Toukoki, C., & Eichenbaum, Z. (2008) · Infection and Immunity · doi:10.1128/iai.00300-08 · Cited by 90 · see citing works
- Authors
- Kocks, C., Gouin, E., Tabouret, M., Berche, P., Ohayon, H., & Cossart, P.
- Affiliations
- Institut Pasteur (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Hôpital Necker-Enfants Malades (FR)
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1992-02
- Volume
- 68
- Issue
- 3
- Pages
- 521-531
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2094070743
- Cited by
- 817
- References
- 50
- Citations by year
- 2026: 11 · 2025: 17 · 2024: 14 · 2023: 19 · 2022: 20 · 2021: 18 · 2020: 26 · 2019: 23 · 2018: 20 · 2017: 20 · 2016: 32 · 2015: 17 · 2014: 32 · 2013: 21 · 2012: 18
- FWCI
- 35.56
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Listeria monocytogenes in Food Safety
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Keywords
- Listeria monocytogenes, actA gene, actin polymerization, bacterial surface proteins, cell-to-cell transmission, intracellular motility, avirulent mutants, gene disruption, operon
- MeSH terms
- Actins, Amino Acid Sequence, Animals, Bacterial Proteins, Base Sequence, Cloning, Molecular, Genes, Bacterial, Listeria monocytogenes, Membrane Proteins, Molecular Sequence Data, Oligonucleotides, Endopeptidases, Phospholipases, Virulence, 3T3 Cells, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review