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Structure of an antibody-antigen complex: crystal structure of the HyHEL-10 Fab-lysozyme complex.
Open accessAbstract
The crystal structure of the complex of the anti-lysozyme HyHEL-10 Fab and hen egg white lysozyme has been determined to a nominal resolution of 3.0 A. The antigenic determinant (epitope) on the lysozyme is discontinuous, consisting of residues from four different regions of the linear sequence. It consists of the exposed residues of an alpha-helix together with surrounding amino acids. The epitope crosses the active-site cleft and includes a tryptophan located within this cleft. The combining site of the antibody is mostly flat with a protuberance made up of two tyrosines that penetrate the cleft. All six complementarity-determining regions of the Fab contribute at least one residue to the binding; one residue from the framework is also in contact with the lysozyme. The contacting residues on the antibody contain a disproportionate number of aromatic side chains. The antibody-antigen contact mainly involves hydrogen bonds and van der Waals interactions; there is one ion-pair interaction but it is weak.
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Citations
Cited by
The structure of protein-protein recognition sites.
Janin, J., & Chothia, C. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)46181-3 · Cited by 819
Paus, D., Phan, T. G., Chan, T. D., Gardam, S., Basten, A., & Brink, R. (2006) · The Journal of Experimental Medicine · doi:10.1084/jem.20060087 · Cited by 567
Structural Evidence for Induced Fit as a Mechanism for Antibody-Antigen Recognition
Rini, J. M., Schulze-Gahmen, U., & Wilson, I. A. (1992) · Science · doi:10.1126/science.1546293 · Cited by 527
Crystal structure of a camel single-domain VH antibody fragment in complex with lysozyme
Desmyter, A., Transue, T. R., Ghahroudi, M. A., Dao Thi, M.-H., Poortmans, F., Hamers, R., Muyldermans, S., & Wyns, L. (1996) · Nature Structural & Molecular Biology · doi:10.1038/nsb0996-803 · Cited by 522
Refined Structure of an Intact IgG2a Monoclonal Antibody,
Harris, L. J., Larson, S. B., Hasel, K. W., & McPherson, A. (1997) · Biochemistry · doi:10.1021/bi962514+ · Cited by 451
Metadata
- Authors
- Padlan, E. A., Silverton, E. W., Sheriff, S., Cohen, G. H., Smith-Gill, S. J., & Davies, D. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-08
- Volume
- 86
- Issue
- 15
- Pages
- 5938-5942
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2064811345
- Cited by
- 458
- References
- 0
- FWCI
- 33.90
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Structure of an antibody-antigen complex: crystal structure of the HyHEL-10 Fab-lysozyme complex. https://app.cochat.ai/papers/10.1073/pnas.86.15.5938
- The paper
- Padlan, E. A., Silverton, E. W., Sheriff, S., Cohen, G. H., Smith-Gill, S. J., & Davies, D. R. (1989). Structure of an antibody-antigen complex: crystal structure of the HyHEL-10 Fab-lysozyme complex. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.15.5938
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
