The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Human neutrophils and mononuclear cells inhibit platelet aggregation by releasing a nitric oxide-like factor.
Open accessAbstract
Incubation of neutrophils or mononuclear cells with washed platelets (all prepared from human venous blood) resulted in an inhibition of thrombin-induced platelet aggregation that was dependent on the number of nucleated cells added. The inhibition was potentiated by superoxide dismutase and reversed by oxyhemoglobin. In the case of neutrophils the inhibition was associated with an increase in cGMP, whereas with mononuclear cells both cAMP and cGMP were increased. The inhibitory activity of neutrophils or mononuclear cells was prevented by their preincubation with NG-monomethyl-L-arginine methyl ester. L-Arginine reversed the action of NG-monomethyl-L-arginine methyl ester, whereas D-arginine was ineffective. Preincubation of the cells with catalase or mannitol did not prevent their inhibitory action on platelet aggregation. The inhibition of platelet aggregation was not due to platelet damage or to uptake of thrombin by neutrophils or mononuclear cells. It was overcome by increasing the concentration of thrombin and was absent in cell-free supernatants obtained from a suspension of neutrophils or mononuclear cells or from mixtures of platelets with neutrophils or platelets with mononuclear cells. These data provide evidence for the release of a nitric oxide-like factor from human neutrophils and mononuclear cells. In addition, evidence is provided that, as in stimulated murine macrophages and endothelial cells, the precursor of this factor is L-arginine.
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Cites
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Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor
Palmer, R. M. J., Ferrige, A. G., & Moncada, S. (1987) · Nature · doi:10.1038/327524a0 · Cited by 10,704
Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide.
Ignarro, L. J., Buga, G. M., Wood, K. S., Byrns, R. E., & Chaudhuri, G. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.24.9265 · Cited by 5,074
Vascular endothelial cells synthesize nitric oxide from L-arginine
Palmer, R. M. J., Ashton, D. S., & Moncada, S. (1988) · Nature · doi:10.1038/333664a0 · Cited by 4,769
Babior, B. M., Kipnes, R. S., & Curnutte, J. T. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107236 · Cited by 2,931
Oxygen-Dependent Microbial Killing by Phagocytes: (First of Two Parts)
Babior, B. M. (1978) · New England Journal of Medicine · doi:10.1056/nejm197803232981205 · Cited by 2,486
Superoxide anion is involved in the breakdown of endothelium-derived vascular relaxing factor
Gryglewski, R. J., Palmer, R. M. J., & Moncada, S. (1986) · Nature · doi:10.1038/320454a0 · Cited by 2,400
Self-citationThe aggregation of blood platelets
Born, G. V. R., & Cross, M. J. (1963) · The Journal of Physiology · doi:10.1113/jphysiol.1963.sp007185 · Cited by 2,111
Nitric oxide: A cytotoxic activated macrophage effector molecule
Hibbs, J. B., Taintor, R. R., Vavrin, Z., & Rachlin, E. M. (1988) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(88)80015-9 · Cited by 2,030
Macrophage oxidation of L-arginine to nitrite and nitrate: nitric oxide is an intermediate
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Cited by
Regulatory Functions of the Vascular Endothelium
Epstein, F. H., Vane, J. R., Änggård, E. E., & Botting, R. M. (1990) · New England Journal of Medicine · doi:10.1056/nejm199007053230106 · Cited by 1,889
Self-citationRole of nitric oxide synthesis in macrophage antimicrobial activity
Nathan, C. F., & Hibbs, J. B. (1991) · Current Opinion in Immunology · doi:10.1016/0952-7915(91)90079-g · Cited by 1,523
Mammalian nitric oxide synthases
Stuehr, D. J. (1999) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(99)00016-x · Cited by 996
A novel neuronal messenger molecule in brain: The free radical, nitric oxide
Dawson, T. M., Dawson, V. L., & Snyder, S. H. (1992) · Annals of Neurology · doi:10.1002/ana.410320302 · Cited by 834
Isoforms of nitric oxide synthase Characterization and purification from different cell types
Förstermann, U., Schmidt, H. H. H. W., Pollock, J. S., Sheng, H., Mitchell, J. A., Warner, T. D., Nakane, M., & Murad, F. (1991) · Biochemical Pharmacology · doi:10.1016/0006-2952(91)90581-o · Cited by 816
Metadata
- Authors
- Salvemini, D., de Nucci, G., Gryglewski, R. J., & Vane, J. R.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-08
- Volume
- 86
- Issue
- 16
- Pages
- 6328-6332
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2004940500
- Cited by
- 307
- References
- 34
- FWCI
- 14.63
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Human neutrophils and mononuclear cells inhibit platelet aggregation by releasing a nitric oxide-like factor. https://app.cochat.ai/papers/10.1073/pnas.86.16.6328
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- Salvemini, D., de Nucci, G., Gryglewski, R. J., & Vane, J. R. (1989). Human neutrophils and mononuclear cells inhibit platelet aggregation by releasing a nitric oxide-like factor. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.16.6328
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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