Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent
Open accessAbstract
As a highly reactive substance produced in biological systems by the one-electron reduction of oxygen, superoxide (O(2) (-)) seemed a likely candidate as a bactericidal agent in leukocytes. The reduction of cytochrome c, a process in which O(2) (-) may serve as an electron donor, was found to occur when the cytochrome was incubated with leukocytes. O(2) (-) was identified as the agent responsible for the leukocyte-mediated reduction of cytochrome c by the demonstration that the reaction was abolished by superoxide dismutase, an enzyme that destroys O(2) (-), but not by boiled dismutase, albumin, or catalase. Leukocyte O(2) (-) production doubled in the presence of latex particles. The average rate of formation of O(2) (-) in the presence of these particles was 1.03 nmol/10(7) cells per 15 min. This rate, however, is only a lower limit of the true rate of O(2) (-) production, since any O(2) (-) which reacted with constituents other than cytochrome c would have gone undetected. Thus. O(2) (-) is made by leukocytes under circumstances which suggest that it may be involved in bacterial killing.
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NOX are mainly located in the plasma membrane.
“NOX are mainly located in the plasma membrane [31,32].”
From Superoxide Anion Chemistry—Its Role at the Core of the Innate Immunity (2023) · cites this paper as [32]
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- Authors
- Babior, B. M., Kipnes, R. S., & Curnutte, J. T.
- Affiliations
- Tufts Medical Center (US)
- Venue
- Journal of Clinical Investigation
- Published
- 1973-03-01
- Volume
- 52
- Issue
- 3
- Pages
- 741-744
- ISSN
- 0021-9738 (ISSN-L)
- PMC
- PMC302313
- OpenAlex
- W2117966002
- Cited by
- 2,931
- References
- 12
- Citations by year
- 2026: 16 · 2025: 18 · 2024: 18 · 2023: 32 · 2022: 25 · 2021: 45 · 2020: 28 · 2019: 36 · 2018: 33 · 2017: 40 · 2016: 49 · 2015: 42 · 2014: 52 · 2013: 52 · 2012: 38
- FWCI
- 19.10
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Journal of Clinical Investigation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
- Keywords
- superoxide, leukocytes, bactericidal activity, cytochrome c reduction, superoxide dismutase, latex particles, reactive oxygen species, bacterial killing, phagocytes
- MeSH terms
- Cytochrome c Group, Humans, Latex, Leukocytes, Microspheres, Oxidation-Reduction, Oxygen, Oxygen Consumption, Superoxide Dismutase
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