The DOI matches its Crossref record; 11 of the 11 references it cites were found in a registry or an index.
Efficient rescue of integrated shuttle vectors from transgenic mice: a model for studying mutations in vivo.
Open accessAbstract
To study gene mutations in different organs and tissues of an experimental animal, we produced transgenic mice harboring bacteriophage lambda shuttle vectors integrated in the genome in a head-to-tail arrangement. As a target for mutagenesis, the selectable bacterial lacZ gene was cloned in the vector. The integrated vectors were rescued from total genomic DNA with high efficiency by in vitro packaging and propagation of the phages in a LacZ- strain of Escherichia coli C. The background mutation frequencies in brain and liver DNA appeared to be low, as was indicated by the absence of colorless plaques among 138,816 and 168,160 phage isolated from brain and liver DNA, respectively. Treatment of adult female transgenic mice with N-ethyl-N-nitrosourea resulted in a dose-dependent increase of the frequency of mutated vectors isolated from brain DNA, up to 7.4 x 10(-5) at 250 mg of the alkylating agent per kilogram of body weight. At this dose, in liver DNA of the same mice, mutation frequencies were approximately 3 x 10(-5). DNA sequence analysis of four mutant vectors isolated from brain DNA indicated predominantly G.C----A.T transitions. These results demonstrate the value of this transgenic mouse model in studying gene mutations in vivo. In addition to its use in fundamental research, the system could be used as a sensitive, organ-specific, short-term mutagenicity assay.
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Differences between germline and somatic mutation rates in humans and mice
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Self-citationThe role of DNA damage and repair in aging through the prism of Koch-like criteria
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Somatic Mutagenesis in Mammals and Its Implications for Human Disease and Aging
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Metadata
- Authors
- Gossen, J. A., de Leeuw, W. J., Tan, C. H., Zwarthoff, E. C., Berends, F., Lohman, P. H., Knook, D. L., & Vijg, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-10
- Volume
- 86
- Issue
- 20
- Pages
- 7971-7975
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1976856900
- Cited by
- 494
- References
- 11
- FWCI
- 5.05
- Type
- journal article
- Language
- en
- Open access
- green
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- Gossen, J. A., de Leeuw, W. J., Tan, C. H., Zwarthoff, E. C., Berends, F., Lohman, P. H., Knook, D. L., & Vijg, J. (1989). Efficient rescue of integrated shuttle vectors from transgenic mice: a model for studying mutations in vivo. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.20.7971
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