Nerve growth factor mRNA and protein increase in hypothalamus in a mouse model of aggression.
Open accessAbstract
The effects of intermale aggressive behavior induced by social isolation on the level of nerve growth factor (NGF) mRNA and protein were investigated in central and peripheral mouse tissues. A large increase in NGF mRNA and protein was observed in hypothalamus, with no changes in cerebral cortex, hippocampus, and cerebellum. No change in NGF mRNA levels was found in heart, spleen, vas deferens, and submaxillary salivary gland. The cellular localization of NGF mRNA in the central nervous system was investigated by in situ hybridization. Numerous nerve cells were specifically labeled in preoptic and ventrolateral nuclei of the hypothalamus, as well as in the cornu ammonis region of the hippocampus and throughout all layers of the cerebral cortex, with the highest concentration in layer III. The present results firmly establish that nerve cells constitute the major source in NGF in the brain. They also open the way to understanding the regulation of NGF biosynthesis in the central nervous system.
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- Authors
- Spillantini, M. G., Aloe, L., Alleva, E., De Simone, R., Goedert, M., & Levi-Montalcini, R.
- Affiliations
- MRC Laboratory of Molecular Biology (GB)
- Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom.
- Venue
- Proceedings of the National Academy of Sciences
- Publisher
- National Academy of Sciences
- Published
- 1989-11
- Volume
- 86
- Issue
- 21
- Pages
- 8555-8559
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- PMC
- PMC298321
- OpenAlex
- W2042727575
- Cited by
- 136
- References
- 43
- Citations by year
- 2025: 3 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 4 · 2017: 2 · 2016: 3 · 2015: 1 · 2014: 2 · 2013: 2 · 2012: 5
- FWCI
- 1.65
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Proceedings of the National Academy of Sciences (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nerve injury and regeneration
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- nerve growth factor, hypothalamus, aggression, social isolation, NGF mRNA, preoptic nucleus, ventrolateral nucleus, hippocampus, cerebral cortex, neurotrophins, mouse model
- MeSH terms
- Aggression, Animals, Brain, Hypothalamus, Male, Models, Psychological, Nerve Growth Factors, Nucleic Acid Hybridization, Organ Specificity, RNA, Messenger, Social Isolation, Transcription, Genetic, Blotting, Northern, Mice
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