The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease.
Open accessAbstract
The KEX2-encoded endoprotease was overproduced in yeast several hundred-fold and further purified to achieve a 10,000-fold enrichment in specific activity. The enzyme was (i) membrane-bound, but solubilized by detergents; (ii) able to cleave peptide substrates at both Lys-Arg and Arg-Arg sites; (iii) inhibited by EDTA and EGTA (but not o-phenanthroline), but fully reactivated by Ca2+; (iv) unaffected by 5-10 mM phenylmethylsulfonyl fluoride, N alpha-(ptosyl)lysine chloromethyl ketone, or L-1-tosylamido-2-phenylethyl chloromethyl ketone, but inactivated by 1-2 microM Ala-Lys-Arg-chloromethyl ketone; (v) labeled specifically by 125I-labeled Tyr-Ala-Lys-Arg-chloromethyl ketone; and (vi) resistant to trans-epoxysuccinate compounds (which inactivate thiol proteases), but inactivated by diisopropyl fluorophosphate (a diagnostic serine protease inhibitor). Mutant enzyme molecules lacking as many as 200 C-terminal residues still retained Ca2+-dependent protease activity and were labeled by 125I-labeled Tyr-Ala-Lys-Arg-chloromethyl ketone.
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Cites
The place of human γ-trace (cystatin C) amongst the cysteine proteinase inhibitors
Barrett, A. J., Davies, M. E., & Grubb, A. (1984) · Biochemical and Biophysical Research Communications · doi:10.1016/0006-291x(84)91302-0 · Cited by 294
Cited by
The biology and therapeutic targeting of the proprotein convertases
Seidah, N. G., & Prat, A. (2012) · Nature Reviews Drug Discovery · doi:10.1038/nrd3699 · Cited by 759
Yeast viral killer toxins: lethality and self-protection
Schmitt, M. J., & Breinig, F. (2006) · Nature Reviews Microbiology · doi:10.1038/nrmicro1347 · Cited by 314
Albrecht, A., Felk, A., Pichova, I., Naglik, J. R., Schaller, M., de Groot, P., MacCallum, D., Odds, F. C., Schäfer, W., Klis, F., et al. (2006) · Journal of Biological Chemistry · doi:10.1074/jbc.m509297200 · Cited by 270
A Homolog of Voltage-Gated Ca 2+ Channels Stimulated by Depletion of Secretory Ca 2+ in Yeast
Locke, E. G., Bonilla, M., Liang, L., Takita, Y., & Cunningham, K. W. (2000) · Molecular and Cellular Biology · doi:10.1128/mcb.20.18.6686-6694.2000 · Cited by 212
Bruinenberg, P. G., De Vos, W. M., & Siezen, R. J. (2000) · Applied and Environmental Microbiology · doi:10.1128/aem.66.7.2859-2865.2000 · Cited by 13
Metadata
- Authors
- Fuller, R. S., Brake, A., & Thorner, J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1989-03
- Volume
- 86
- Issue
- 5
- Pages
- 1434-1438
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2093508320
- Cited by
- 392
- References
- 1
- FWCI
- 11.16
- Type
- journal article
- Language
- en
- Open access
- green
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- Fuller, R. S., Brake, A., & Thorner, J. (1989). Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.5.1434
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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