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The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification.

Open access
Pääbo, S·1989·Proceedings of the National Academy of Sciences

Abstract

Several chemical and enzymatic properties were examined in the DNA extracted from dry remains of soft tissues that vary in age from 4 to 13,000 years and represent four species, including two extinct animals (the marsupial wolf and giant ground sloth). The DNA obtained was invariably of a low average molecular size and damaged by oxidative processes, which primarily manifest themselves as modifications of pyrimidines and sugar residues as well as baseless sites and intermolecular cross-links. This renders molecular cloning difficult. However, the polymerase chain reaction can be used to amplify and study short mitochondrial DNA sequences that are of anthropological and evolutionary significance. This opens up the prospect of performing diachronical studies of molecular evolutionary genetics.

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Crossref · 10.1073/pnas.86.6.1939

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Metadata

Authors
Pääbo, S.
Venue
Proceedings of the National Academy of Sciences
Published
1989-03
Volume
86
Issue
6
Pages
1939-1943
ISSN
  • 0027-8424 (ISSN-L)
  • 1091-6490
OpenAlex
W2090684975
Cited by
1,011
References
3
FWCI
14.66
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification. https://app.cochat.ai/papers/10.1073/pnas.86.6.1939
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Pääbo, S. (1989). Ancient DNA: extraction, characterization, molecular cloning, and enzymatic amplification. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.86.6.1939

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.