The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Negative growth regulation in a glioblastoma tumor cell line that conditionally expresses human wild-type p53.
Open accessAbstract
To investigate the effect that human wild-type p53 (wt-p53) expression has on cell proliferation we constructed a recombinant plasmid, pM47, in which wt-p53 cDNA is under transcriptional control of the hormone-inducible mouse mammary tumor virus promoter linked to the dominant biochemical selection marker gene Eco gpt. The pM47 plasmid was introduced into T98G cells derived from a human glioblastoma multiforme tumor, and a stable clonal cell line, GM47.23, was derived that conditionally expressed wt-p53 following exposure to dexamethasone. We show that induction of wt-p53 expression in exponentially growing cells inhibits cell cycle progression and that the inhibitory effect is reversible upon removal of the inducer or infection with simian virus 40. Moreover, when growth-arrested cells are stimulated to proliferate, induction of wt-p53 expression inhibits G0/G1 progression into S phase and the cells accumulate with a DNA content equivalent to cells arrested in the G0/G1 phase of the cell cycle. Taken together, these studies suggest that wt-p53 may play a negative role in growth regulation.
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Cites
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Cited by
WAF1, a potential mediator of p53 tumor suppression
El-Deiry, W. S., Tokino, T., Velculescu, V. E., Levy, D. B., Parsons, R., Trent, J. M., Lin, D., Mercer, W. E., Kinzler, K. W., & Vogelstein, B. (1993) · Cell · doi:10.1016/0092-8674(93)90500-p · Cited by 8,321
Self-citationp53 Mutations in Human Cancers
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Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumours
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Tumor suppressor p53 is a direct transcriptional activator of the human bax gene
Toshiyuki, M., & Reed, J. C. (1995) · Cell · doi:10.1016/0092-8674(95)90412-3 · Cited by 4,063
p53 is required for radiation-induced apoptosis in mouse thymocytes
Lowe, S. W., Schmitt, E. M., Smith, S. W., Osborne, B. A., & Jacks, T. (1993) · Nature · doi:10.1038/362847a0 · Cited by 2,864
Metadata
- Authors
- Mercer, W. E., Shields, M. T., Amin, M., Sauve, G. J., Appella, E., Romano, J. W., & Ullrich, S. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-08
- Volume
- 87
- Issue
- 16
- Pages
- 6166-6170
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2040549178
- Cited by
- 477
- References
- 38
- FWCI
- 20.91
- Type
- journal article
- Language
- en
- Open access
- green
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- Mercer, W. E., Shields, M. T., Amin, M., Sauve, G. J., Appella, E., Romano, J. W., & Ullrich, S. J. (1990). Negative growth regulation in a glioblastoma tumor cell line that conditionally expresses human wild-type p53. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.16.6166
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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