The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
Ligand-affinity cloning and structure of a cell surface heparan sulfate proteoglycan that binds basic fibroblast growth factor.
Open accessAbstract
Expression cloning of cDNAs encoding a basic fibroblast growth factor (FGF) binding protein confirms previous hypotheses that this molecule is a cell-surface heparan sulfate proteoglycan. A cDNA library constructed from a hamster kidney cell line rich in FGF receptor activity was transfected into a human lymphoblastoid cell line. Clones expressing functional basic FGF binding proteins at their surfaces were enriched by panning on plastic dishes coated with human basic FGF. The amino acid sequence deduced from the isolated cDNAs revealed several interesting features, including hydrophobic signal and transmembrane domains that flank an extracellular region containing six potential attachment sites for glycosaminoglycan side chains. The structure also contains a short hydrophilic cytoplasmic tail sequence homologous to previously reported actin binding domains. Binding of basic FGF to cells expressing the binding protein could be inhibited by heparin and heparan sulfate but not by chondroitin sulfate, dermatan sulfate, or keratan sulfate. In addition to binding basic FGF, this protein or related surface proteins may function as an initial cellular attachment site for other growth factors and for viruses, such as herpes simplex virus.
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Cited by
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Requirement of Heparan Sulfate for bFGF-Mediated Fibroblast Growth and Myoblast Differentiation
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Structural and Functional Diversity in the FGf Receptor Multigene Family
(1992) · Advances in Cancer Research · doi:10.1016/s0065-230x(08)60821-0 · Cited by 1,250
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Metadata
- Authors
- Kiefer, M. C., Stephans, J. C., Crawford, K., Okino, K., & Barr, P. J.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1990-09
- Volume
- 87
- Issue
- 18
- Pages
- 6985-6989
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W1992486637
- Cited by
- 271
- References
- 44
- FWCI
- 18.98
- Type
- journal article
- Language
- en
- Open access
- green
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- Kiefer, M. C., Stephans, J. C., Crawford, K., Okino, K., & Barr, P. J. (1990). Ligand-affinity cloning and structure of a cell surface heparan sulfate proteoglycan that binds basic fibroblast growth factor. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.87.18.6985
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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